The signaling pathways linking to lysophosphatidic acid-promoted meiotic maturation in mice
The signaling pathways linking to lysophosphatidic acid-promoted meiotic maturation in mice
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DOI：
10.1016/j.lfs.2006.01.028
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发表时间：
2006-06-27
期刊：
影响因子：
6.1
通讯作者：
Irahara, Minoru
中科院分区：
文献类型：
--
作者：
Komatsu, Junko;Yamano, Shuji;Irahara, Minoru
研究了溶血磷脂酸促进小鼠减数分裂成熟的信号通路。用10(-5)M溶血磷脂酸（LPA组）培养小鼠卵母细胞-卵丘细胞复合物时，卵母细胞核成熟率显著提高。百日咳毒素、染料木黄酮、U73122、Ro320432、PD98059或SB203580的添加显著抑制溶血磷脂酸刺激核成熟率的升高。这些结果表明，Gi/o偶联溶血磷脂酸受体激活磷脂酰肌醇特异性磷脂酶C，并导致ERK和MAP激酶激活，这是由二酰基甘油依赖性蛋白激酶C触发的。LPA组细胞内cAMP浓度显著低于对照组（0.117 +/- 0.04 fmol/卵母细胞vs. 0.176 0.036 fmol/卵母细胞，p < 0.05）。综上所述，我们的研究结果表明，溶磷脂酸通过小鼠卵丘细胞表面的gi蛋白连接受体刺激磷脂酶C，并刺激细胞外信号调节激酶和p38丝裂原活化激酶，导致卵丘细胞和卵母细胞之间的间隙连接关闭或松动。由此导致的卵母细胞cAMP水平的早期降低可能促进体外小鼠卵母细胞的核成熟。(c) 2006爱思唯尔公司版权所有。
The signaling pathways linking to lysophosphatidic acid-promoted meiotic maturation in mice were studied. When mouse oocyte-cumulus cells complexes were cultured with 10(-5) M lysophosphatidic acid (the LPA group), the rate of oocyte nuclear maturation was significantly increased. Additions of pertussis toxin, genistein, U73122, Ro320432, PD98059 or SB203580 significantly suppressed the increase in lysophosphatidic acid-stimulated nuclear maturation rate. These results suggested that Gi/o-coupled lysophosphatidic acid receptors activate phosphatidylinositol-specific phospholipase C, and result in ERK and MAP kinase activation, which is triggered by diacylglycerol-dependent protein kinase C. When intracellular cAMP concentrations of oocytes in the LPA and control groups were measured using the acetylation assay, the intracellular cAMP concentration of an oocyte in the LPA group was significantly lower than the control oocyte (0.117 +/- 0.04 fmol/oocyte vs. 0.176 0.036 fmol/oocyte, p < 0.05). In conclusion, our results suggested that lysophosphatidic acid stimulates phospholipase C through a Gi-protein linked receptor on the surface of mouse cumulus cells and stimulates both extracellular signal-regulated kinase and p38 mitogen-activated kinase, resulting in the closure or loose of gap junctions between cumulus cells and the oocyte. The resultant early decrease of oocyte cAMP levels may promote nuclear maturation of mouse oocytes in vitro. (c) 2006 Elsevier Inc. All rights reserved.