Interaction of N-trifluoroacetyladriamycin-14-O-hemiadipate with chicken leukemia RNA polymerase. Formation of drug-enzyme complex.
Interaction of N-trifluoroacetyladriamycin-14-O-hemiadipate with chicken leukemia RNA polymerase. Formation of drug-enzyme complex.
复制标题
N-三氟乙酰阿霉素-14-O-半己二酸与鸡白血病 RNA 聚合酶的相互作用。
DOI：
10.1016/0006-2952(86)90274-1
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发表时间：
1986
影响因子：
5.8
通讯作者：
Israel,M
中科院分区：
文献类型：
--
作者：
Chuang,RY;Chuang,LF;Israel,M
The biochemical mechanism of theN-trifluoroacetyladriamycin-14-O-hemiadipate-induced inhibition of RNA synthesisin vitroby chicken (myeloblastosis) leukemia RNA polymerase II was studied. The inhibition was found to be dependent upon preincubation of the drug with the enzyme prior to enzyme assays, suggesting that drug-enzyme interactions occur. A drug-enzyme association complex was subsequently isolated through glycerol gradient sedimentation and further characterized by fluorescent microscopic studies. The drug was dissociated from the complex upon sodium dodecyl sulfate (SDS)-gel electrophoresis, revealing the non-covalent nature of the binding between the drug and the RNA polymerase.
