Molecular characterization of a canine respiratory coronavirus strain detected in Italy
Molecular characterization of a canine respiratory coronavirus strain detected in Italy
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DOI：
10.1016/j.virusres.2008.12.011
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发表时间：
2009-04-01
期刊：
影响因子：
5
通讯作者：
Decaro, Nicola
中科院分区：
文献类型：
--
作者：
Lorusso, Alessio;Desario, Costantina;Decaro, Nicola
冠状病毒（CoV）是正链、非节段RNA病毒，通常会导致人类、伴侣动物和牲畜出现呼吸道和肠道疾病。它们通过基因重组和/或点突变进化的倾向被认识到，从而产生具有不同组织或宿主嗜性的新病毒基因型和突变体。特别是，一些2a组冠状病毒，包括犬呼吸道冠状病毒（CRCoV），可能起源于严格相关的牛冠状病毒（BCoV）或共同祖先。在这项研究中，我们报告了意大利CRCoV，株240/05的病毒RNA 3 '端的序列分析，以及与现存的牛样病毒，包括先前描述的唯一CRCoV株4182的序列比较。有趣的是，尽管结构蛋白显示出与CRCoV 4182相同的特征，但CRCoV 240/05的刺突蛋白和包膜蛋白基因之间的基因组区域编码三种不同的产物，包括等同的牛4.9kDa非结构蛋白和截短形式的4.8kDa蛋白，而CRCoV 4182具有独特的8.8kDa蛋白。(C)2008 Elsevier B. V.保留所有权利。
Coronaviruses (CoVs) are positive-stranded, non-segmented RNA viruses generally responsible for the emergence of respiratory and enteric disease in humans, companion animals and livestock. Their aptitude to evolve by genetic recombination and/or point mutation is recognized, thus giving rise to new viral genotypes and mutants with different tissues or host tropism. In particular, a probable origin from the strictly related bovine coronavirus (BCoV) or, alternatively, from a common ancestor has been suggested for some group 2a CoVs, including canine respiratory coronavirus (CRCoV). In this study, we report the sequence analysis of the viral RNA 3'-end of an Italian CRCoV, strain 240/05, together with the sequence comparison with extant bovine-like viruses including the sole CRCoV strain 4182 previously described. interestingly, although the structural proteins show the same features of CRCoV 4182, the genomic region between the spike and the envelope protein genes of CRCoV 240/05 encodes for three distinct products, including the equivalent bovine 4.9 kDa non-structural protein and a truncated form of the 4.8 kDa protein, whereas CRCoV 4182 has a unique 8.8 kDa protein. (C) 2008 Elsevier B.V. All rights reserved.