N-glycans harboring the Lewis a epitope are expressed at the surface of plant cells
N-glycans harboring the Lewis a epitope are expressed at the surface of plant cells
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DOI：
10.1046/j.1365-313x.1997.12061411.x
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发表时间：
1997-12-01
期刊：
影响因子：
7.2
通讯作者：
Faye, L
中科院分区：
文献类型：
--
作者：
Fitchette-Lainé, AC;Gomord, V;Faye, L
在植物中，N-连接聚糖在高尔基体中加工成含有β（1，2）-木糖和/或α（1，3）-岩藻糖残基的有限大小的复合型N-聚糖。较大的单触角和双触角N-连接复合聚糖并不常见。本研究重新检查了这种植物N-连接聚糖的结构，并且通过免疫学和结构数据，显示触角由刘易斯a（Le（a））抗原组成，其包含碳水化合物序列Gal β 1-3[Fuc α 1-4]GlcNAc。此外，在西卡莫尔细胞中检测到参与该抗原生物合成的岩藻糖基转移酶活性。这是植物中刘易斯抗原的首次表征，该抗原通常存在于哺乳动物的细胞表面糖缀合物上，并参与识别和粘附过程。含Le（a）的N-连接聚糖在植物中广泛分布，并在细胞表面高度表达，这可能表明其在细胞/细胞通讯中具有推定的功能。
In plants, N-linked glycans are processed in the Golgi apparatus to complex-type N-glycans of limited size containing a beta(1,2)-xylose and/or an alpha(1,3)-fucose residue. Larger mono-and bi-antennary N-linked complex glycans have not often been described. This study has re-examined the structure of such plant N-linked glycans, and, through both immunological and structural data, it is shown that the antennae are composed of Lewis a (Le(a)) antigens, comprising the carbohydrate sequence Gal beta 1-3[Fuc alpha 1-4]GlcNAc. Furthermore, a fucosyltransferase activity involved in the biosynthesis of this antigen was detected in sycamore cells. This is the first characterization in plants of a Lewis antigen that is usually found on cell-surface glycoconjugates in mammals and involved in recognition and adhesion processes. Le(a)-containing N-linked glycans are widely distributed in plants and highly expressed at the cell surface, which may suggest a putative function in cell/cell communication.