WSL5, a pentatricopeptide repeat protein, is essential for chloroplast biogenesis in rice under cold stress
WSL5, a pentatricopeptide repeat protein, is essential for chloroplast biogenesis in rice under cold stress
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DOI：
10.1093/jxb/ery214
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发表时间：
2018-07-20
影响因子：
6.9
通讯作者：
Wan, Jianmin
中科院分区：
文献类型：
--
作者：
Liu, Xi;Lan, Jie;Wan, Jianmin
叶绿体在植物的生长发育中起着重要的作用，低温条件影响叶绿体的发育。虽然许多参与叶绿体生物发生和发育的基因或调控因子已被分离和鉴定，但在寒冷条件下影响叶绿体生物发生的许多其他组分尚未被鉴定。在这里，我们报告了水稻白色条纹叶5（wsl5）突变体的功能特性。该突变体在早期叶片发育过程中形成白色条纹叶，并且在冷胁迫下种植时是白化的。遗传和分子分析表明，WSL 5编码一种新的叶绿体靶向五肽重复蛋白。RNA测序分析表明，核编码的光合基因在突变体中的表达被显着抑制，许多叶绿体编码的基因的表达也显着改变。值得注意的是，wsl5突变导致rpl2和atpA的编辑缺陷以及rpl2和rps12的剪接缺陷。在低温胁迫下，WSL 5在叶绿体核糖体生物合成中受到损伤。我们建议，WSL 5等位基因是所需的正常叶绿体发育在维持逆行信号从质体到细胞核在冷胁迫下。
Chloroplasts play an essential role in plant growth and development, and cold conditions affect chloroplast development. Although many genes or regulators involved in chloroplast biogenesis and development have been isolated and characterized, many other components affecting chloroplast biogenesis under cold conditions have not been characterized. Here, we report the functional characterization of a white stripe leaf 5 (wsl5) mutant in rice. The mutant develops white-striped leaves during early leaf development and is albinic when planted under cold stress. Genetic and molecular analysis revealed that WSL5 encodes a novel chloroplast-targeted pentatricopeptide repeat protein. RNA sequencing analysis showed that expression of nuclear-encoded photosynthetic genes in the mutant was significantly repressed, and expression of many chloroplast-encoded genes was also significantly changed. Notably, the wsl5 mutation causes defects in editing of rpl2 and atpA, and splicing of rpl2 and rps12. wsl5 was impaired in chloroplast ribosome biogenesis under cold stress. We propose that the WSL5 allele is required for normal chloroplast development in maintaining retrograde signaling from plastids to the nucleus under cold stress.