LOCATION OF A PROTEIN OF THE FODRIN-SPECTRIN-TW260/240 FAMILY IN THE MOUSE INTESTINAL BRUSH-BORDER
LOCATION OF A PROTEIN OF THE FODRIN-SPECTRIN-TW260/240 FAMILY IN THE MOUSE INTESTINAL BRUSH-BORDER
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DOI：
10.1016/0092-8674(83)90080-6
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发表时间：
1983-01-01
期刊：
影响因子：
64.5
通讯作者：
WILLARD, M
中科院分区：
文献类型：
--
作者：
HIROKAWA, N;CHENEY, RE;WILLARD, M
fodrin-spectrin-TW260/240 （FST）家族的一种蛋白显然是薄原纤维（.apprx）的组成部分。5纳米宽，100-200纳米长)，将肌动蛋白丝束与相邻的肌动蛋白束和肠上皮刷状缘末端网的质膜交联。将分离的毛刷边缘与抗fodrin抗体孵育，并通过速冻、深蚀刻技术制备EM，这些。抗体特异性修饰5nm原纤维。此外，当从毛刷边缘提取抗fodrin反应蛋白时，这些交联原纤维消失。FST显然是由。apprx组成的交联系统的一个组成部分。5nm的原纤维在形态上与。apprx。8 nm含肌球蛋白原纤维，经抗肌球蛋白修饰鉴定。除了将肌动蛋白束与相邻的肌动蛋白束和质膜连接外，这些FST原纤维还可以介导肌动蛋白-囊泡、肌动蛋白-中间丝和囊泡-质膜连接。
A protein of the fodrin-spectrin-TW260/240 (FST) family is apparently a component of the thin fibrils (.apprx. 5 nm wide, 100-200 nm long) that cross-link bundles of actin filaments to adjacent actin bundles and to the plasma membrane in the terminal web of the brush border of the intestinal epithelium. When isolated brush borders were incubated with anti-fodrin antibodies and prepared for EM by the quick-freeze, deep-etch technique, these .apprx. 5 nm fibrils were specifically decorated with the antibody. In addition, these cross-linking fibrils disappeared when the anti-fodrin-reactive proteins were extracted from the brush border. FST apparently is a component of a cross-linking system composed of .apprx. 5 nm fibrils that are morphologically distinct from the .apprx. 8 nm myosin-containing fibrils which were identified by antimyosin decoration. In addition to linking actin bundles to adjacent actin bundles and to the plasma membrane, these FST fibrils may mediate actin-vesicle, actin-intermediate filament and vesicle-plasma membrane linkages.