Lithium promotes proliferation and suppresses migration of Schwann cells.
Lithium promotes proliferation and suppresses migration of Schwann cells.
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DOI：
10.4103/1673-5374.280324
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发表时间：
2020-10
影响因子：
6.1
通讯作者：
Xu H
中科院分区：
文献类型：
--
作者：
Gu XK;Li XR;Lu ML;Xu H
周围神经系统损伤后雪旺细胞的增殖、迁移和再生轴突的再髓鞘形成有助于再生。锂可促进雪旺细胞的髓鞘再生，并促进周围神经再生。然而，锂是否调节雪旺细胞的其他表型，特别是它们的增殖和迁移仍然不清楚。在本研究中，从大鼠坐骨神经残端原代培养雪旺细胞，并将其暴露于0、5、10、15或30 mM氯化锂(LiCl)中24小时。采用细胞计数试剂盒-8，5-乙炔基-2‘-脱氧尿嘧啶核苷、Transwell和伤口愈合试验检测LiCl对雪旺细胞增殖和迁移的影响。细胞计数Kit-8和5-乙炔基-2‘-脱氧尿嘧啶核苷检测显示，5、10、15和30 mM LiCl可显著提高雪旺细胞的存活率和增殖率。基于Transwell的迁移实验和伤口愈合实验表明，10、15和30 mM的LiCl3抑制雪旺细胞的迁移能力。此外，LiCl对雪旺细胞增殖和迁移表型的影响大多呈剂量依赖性。这些数据表明锂处理显著促进了雪旺细胞的增殖，抑制了雪旺细胞的迁移能力。这一结论将为促进周围神经修复和再生的策略提供参考。本研究所有动物实验均于2017年3月2日经南通大学实验动物中心管理委员会中国伦理批复(批准号20170320-017)。
Schwann cell proliferation, migration and remyelination of regenerating axons contribute to regeneration after peripheral nervous system injury. Lithium promotes remyelination by Schwann cells and improves peripheral nerve regeneration. However, whether lithium modulates other phenotypes of Schwann cells, especially their proliferation and migration remains elusive. In the current study, primary Schwann cells from rat sciatic nerve stumps were cultured and exposed to 0, 5, 10, 15, or 30 mM lithium chloride (LiCl) for 24 hours. The effects of LiCl on Schwann cell proliferation and migration were examined using the Cell Counting Kit-8, 5-ethynyl-2′-deoxyuridine, Transwell and wound healing assays. Cell Counting Kit-8 and 5-ethynyl-2′-deoxyuridine assays showed that 5, 10, 15, and 30 mM LiCl significantly increased the viability and proliferation rate of Schwann cells. Transwell-based migration assays and wound healing assays showed that 10, 15, and 30 mM LiCl suppressed the migratory ability of Schwann cells. Furthermore, the effects of LiCl on the proliferation and migration phenotypes of Schwann cells were mostly dose-dependent. These data indicate that lithium treatment significantly promotes the proliferation and inhibits the migratory ability of Schwann cells. This conclusion will inform strategies to promote the repair and regeneration of peripheral nerves. All of the animal experiments in this study were ethically approved by the Administration Committee of Experimental Animal Center of Nantong University, China (approval No. 20170320-017) on March 2, 2017.