ABA insensitivity and low ABA levels during seed development of non-dormant wheat mutants
ABA insensitivity and low ABA levels during seed development of non-dormant wheat mutants
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DOI：
10.1093/jxb/48.7.1415
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发表时间：
1997-07-01
影响因子：
6.9
通讯作者：
Noda, K
中科院分区：
文献类型：
--
作者：
Kawakami, N;Miyake, Y;Noda, K
从小麦红粒系Kitakei-1354（Kitakei）的甲基磺酸乙酯处理群体中分离到3个成熟期无休眠的突变体。从同一株M-2植株的分离世代中筛选出3个突变体（EH 47 -1、EH 47 -2-5和EH 47 -2-6），它们在形态和生理特性上存在差异，表明这些突变体除了不休眠外，还含有多个突变。尽管存在这些差异，但所有突变体的胚在种子成熟的后半期都迅速丧失了对脱落酸（阿坝）的敏感性，而Kitakei胚在成熟后仍保持着对ABA的敏感性。这些结果表明，胚对阿坝的敏感性在种子休眠中起着关键作用。EH 47 -1种子发育过程中胚的阿坝含量变化与北藤相似，但在授粉后30 d显著低于北藤。讨论了在DAP 30时阿坝水平的降低与种子休眠和胚对阿坝敏感性的关系。EH 47 -1与Kitakei杂交后代中的非休眠分离率表明，EH 47 -1的非休眠是一个单一的显性突变。
Three wheat (Triticum aestivum L,) mutants that lacked dormancy at maturity were isolated from an ethylmethane sulphonate-treated population of a dormant red-grained line, Kitakei-1354 (Kitakei). The three mutants (EH47-1, EH47-2-5 and EH47-2-6) were selected in segregating generations derived from one M-2 plant, They differ in morphological and physiological characteristics, showing that these mutants contained several mutations besides non-dormancy. Despite these differences, embryos of all the mutants rapidly lost sensitivity to abscisic acid (ABA) during the later half of seed maturation while Kitakei embryos maintained the sensitivity even after maturity, These results suggest that embryo sensitivity to ABA plays a key role in seed dormancy. The profile of ABA content of EH47-1 embryos during seed development was similar to that of Kitakei, except for a significantly lower level at 30 d after pollination (DAP). This reduced level of ABA at DAP30 is discussed in relation to the development of seed dormancy and ABA sensitivity of the embryos. Segregation ratios for non-dormancy in progeny of EH47-1 x Kitakei crosses suggest that the non-dormancy of EH47-1 is a single dominant mutation.