EXOCRINE CONTAMINATION IMPAIRS IMPLANTATION OF PANCREATIC-ISLETS TRANSPLANTED BENEATH THE KIDNEY CAPSULE
EXOCRINE CONTAMINATION IMPAIRS IMPLANTATION OF PANCREATIC-ISLETS TRANSPLANTED BENEATH THE KIDNEY CAPSULE
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DOI：
10.1016/0022-4804(88)90193-x
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发表时间：
1988-11-01
影响因子：
2.2
通讯作者：
MORRIS, PJ
中科院分区：
文献类型：
--
作者：
GRAY, DWR;SUTTON, R;MORRIS, PJ
通过将单纯或外分泌污染的人、猴、大鼠胰岛分别移植到裸鼠、猴、大鼠肾被膜下，观察外分泌污染对移植肾被膜下胰岛的影响。由于组织坏死和随后的纤维化，外分泌污染导致的外观提示胰岛植入受损。在精选DA大鼠胰岛移植于正常DA大鼠肾被膜下的同种异体胰岛移植模型中，定量检测外分泌污染的影响。这些胰岛要么是纯的，要么是故意被外分泌组织重新污染的(50%或90%的污染)。将400个纯胰岛放置在一个肾被膜下，将400个大小相近的胰岛(来自相同的胰岛制剂)污染，然后放置在对侧肾被膜下。2周后取出肾脏，测定胰岛素含量。受50%或90%外分泌组织污染的肾脏的胰岛素含量明显低于生下纯胰岛的对侧肾脏。这些发现支持这样的观点，即胰岛的外分泌污染导致胰岛移植到受限部位时，如肾被膜下，会导致胰岛植入受损。
The effect of exocrine contamination on islets implanted under the kidney capsule has been studied by histological examination of pure or exocrine-contamination human, monkey, or rat islets transplanted to the kidney capsule of the nude rat, monkey, or rat, respectively. Exocrine contamination resulted in an appearance suggestive of impaired islet implantation, due to tissue necrosis and subsequent fibrosis. The effect of exocrine contamination was examined quantitatively in a rat islet isograft model in which handpicked DA rat islets were transplanted under the kidney capsule of normal DA rats. The islets were either pure or deliberately recontaminated with exocrine tissue (50 or 90% contamination). Four hundred pure islets were placed under one kidney capsule and 400 islets (of similar size and from the same islet preparation) were contaminated and then placed under the contralateral kidney capsule. After 2 weeks the kidneys were removed and extracted for insulin content. The insulin content of kidneys bearing islets contaminated by either 50 or 90% exocrine tissue was significantly reduced when compared to the contralateral kidney bearing pure islets. These findings support the view that exocrine contamination of islets resulted in impaired islet implantation when transplanted to a confined site such as the kidney subcapsule.