CXCL14 enhances insulin-dependent glucose uptake in adipocytes and is related to high-fat diet-induced obesity
CXCL14 enhances insulin-dependent glucose uptake in adipocytes and is related to high-fat diet-induced obesity
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DOI：
10.1016/j.bbrc.2007.10.120
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发表时间：
2007-12-28
影响因子：
3.1
通讯作者：
Chihara, Kazuo
中科院分区：
文献类型：
--
作者：
Takahashi, Michiko;Takahashi, Yutaka;Chihara, Kazuo
越来越多的证据表明肥胖和脂肪组织炎症之间存在联系。趋化因子参与炎症状态的调节。趋化因子（C-X-C基序）配体14（CXCL 14）已知是单核细胞和树突状细胞的化学引诱物。最近，据报道，CXCL 14缺陷小鼠显示出对高脂饮食诱导的肥胖的抵抗力。在这项研究中，我们确定CXCL 14作为生长激素（GH）诱导的基因在HepG 2肝癌细胞。在脂肪组织和肝脏中检测到CXCL 14的大量体内表达。其表达和分泌显著增加胰岛素管理和高脂饮食。有趣的是，3 T3-L1脂肪细胞与CXCL 14的孵育刺激胰岛素依赖性葡萄糖摄取。此外，这种作用与增强的胰岛素信号传导有关。CXCL 14增强胰岛素诱导的胰岛素受体和胰岛素受体底物-1的酪氨酸磷酸化。这些结果表明，CXCL 14在高脂饮食诱导的肥胖中起着因果作用。(C)2007年爱思唯尔公司All rights reserved.
Accumulating evidence suggests an association between obesity and adipose tissue inflammation. Chemokines are involved in the regulation of inflammation status. Chemokine (C-X-C motif) ligand 14 (CXCL14) is known to be a chemoattractant for monocyte and dendritic cells. Recently, it was reported that CXCL14-deficient mice show resistance to high-fat diet-induced obesity. In this study, we identified CXCL14 as a growth hormone (GH)-induced gene in HepG2 hepatoma cells. Substantial in vivo expression of CXCL14 was detected in the adipose tissue and liver. Its expression and secretion were strikingly increased by insulin administration and high-fat diet. Intriguingly, incubation of 3T3-L1 adipocytes with CXCL14 stimulated insulin-dependent glucose uptake. Further, this effect was associated with enhanced insulin signaling. CXCL14 enhanced the insulin-induced tyrosine phosphorylation of insulin receptors and insulin receptor substrate-1. These results suggest that CXCL14 plays a causal role in high-fat diet-induced obesity. (C) 2007 Elsevier Inc. All rights reserved.