Global DNA methylation evaluation: potential complementary marker in differential diagnosis of thyroid neoplasia
Global DNA methylation evaluation: potential complementary marker in differential diagnosis of thyroid neoplasia
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DOI：
10.1007/s00428-005-1268-5
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发表时间：
2005-07-01
期刊：
影响因子：
3.5
通讯作者：
Peoc'h, M
中科院分区：
文献类型：
--
作者：
Galusca, B;Dumollard, JM;Peoc'h, M
全球DNA低甲基化的影响，最近报道在几个模型的肿瘤发生。关于甲状腺肿瘤的表观遗传学事件知之甚少。该研究旨在使用5-甲基胞苷（5-mc）特异性单克隆抗体评估几种类型甲状腺肿瘤的整体DNA甲基化状态，并确定该标记物的诊断潜力。对17例乳头状甲状腺癌（PTC）、6例滤泡性甲状腺癌（FTC）、16例滤泡性腺瘤（FA）、19例结节性甲状腺肿（NG）和10例Hurthle细胞腺瘤（HCA）进行5-mc免疫组化评分。还评估了半乳糖凝集素-3的表达。计算机图像分析显示，甲状腺癌组织中5-mc免疫染色水平明显低于甲状腺良性肿瘤或癌旁正常甲状腺组织（P < 0.0001）。总体而言，5-mc鉴别甲状腺良恶性肿瘤的准确性与galectin-3相似（89%对87%，P > 0.05）。5-mc与半乳糖凝集素-3的组合导致96%的优异准确度水平。在滤泡性肿瘤中，5-mc鉴别恶性肿瘤的准确性倾向于高于galectin-3（90%对66%，P=0.06）。这些数据强调了甲状腺结节中表观遗传事件评估的必要性，并建议将整体DNA甲基化评估作为一种潜在的诊断工具，与其他有价值的标记物联合收割机相结合。
The implications of global DNA hypomethylation were recently reported in several models of tumorigenesis. Little is known about this epigenetic event in thyroid neoplasia. The study aimed to evaluate the status of global DNA methylation in several types of thyroid tumors using a monoclonal antibody specific for 5-methylcytidine (5-mc) and to define the diagnosis potential of this marker. 5-mc immunostaining scores were calculated in 17 papillary thyroid carcinomas (PTC), 6 follicular thyroid carcinomas (FTC), 16 follicular adenomas (FA), 19 nodular goiters (NG) and ten Hurthle cells adenomas (HCA). The expression of galectin-3 was also evaluated. Computerized image analysis showed a significant lower level of 5-mc immunostaining in thyroid carcinoma when compared with benign tumors or adjacent normal thyroid parenchyma (P < 0.0001). Overall, 5-mc accuracy to distinguish malign from benign thyroid tumors was similar to that of galectin-3 (89% versus 87%, P > 0.05). The combination of 5-mc with galectin-3 led to an excellent accuracy level of 96%. Among follicular neoplasia 5-mc accuracy to differentiate malign tumors trends to be higher than galectin-3 one (90% versus 66%, P=0.06). These data stress the necessity of epigenetic events evaluation among thyroid nodules and propose global DNA methylation assessment as a potential diagnostic tool to combine with other valuable markers.