Peptide-based fluorescent sensors of protein kinase activity: Design and applications
Peptide-based fluorescent sensors of protein kinase activity: Design and applications
复制标题
DOI：
10.1016/j.bbapap.2007.07.016
复制
发表时间：
2008-01-01
影响因子：
3.2
通讯作者：
Lawrence, David S.
中科院分区：
文献类型：
--
作者：
Shanna, Vyas;Wang, Qunzhao;Lawrence, David S.
蛋白激酶通过细胞信号通路控制信息流。对它们行为的详细分析增强了我们理解正常细胞状态和设计疾病治疗干预措施的能力。介绍了用于蛋白激酶活性研究的“环境敏感”、“深度猝灭”和“自报告”传感器系统的设计和应用。这些传感器允许以连续的方式对各种激酶进行实时活性测量。由于这些传感器可以从体外筛选到活细胞中的激酶活性成像，因此它们支持药物发现的初期和后期阶段。(C)2007 Elsevier B.V.保留所有权利。
Protein kinases control the flow of information through cell-signaling pathways. A detailed analysis of their behavior enhances our ability to understand normal cellular states and to devise therapeutic interventions for diseases. The design and application of "Environmentally-Sensitive", "Deep-Quench" and "Self-Reporting" sensor systems for studying protein kinase activity are described. These sensors allow real-time activity measurements in a continuous manner for a wide variety of kinases. As these sensors can be adapted from an in vitro screen to imaging kinase activity in living cells, they support both preliminary and later stages of drug discovery. (C) 2007 Elsevier B.V. All rights reserved.