Improved dilute bicelle solutions for high-resolution NMR of biological macromolecules
Improved dilute bicelle solutions for high-resolution NMR of biological macromolecules
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DOI：
10.1023/a:1008302110884
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发表时间：
1998-10-01
影响因子：
2.7
通讯作者：
Prestegard, JH
中科院分区：
文献类型：
--
作者：
Losonczi, JA;Prestegard, JH
将生物大分子溶解在稀释的双胞溶液中，其在磁场存在下形成取向的液晶，允许测量各向异性自旋相互作用，例如偶极耦合[Tjandra，N.和Bax，A.，Science，278，1111-1114].然而，这些样品的取向的寿命和温度范围严重依赖于样品组成和实验条件。本文表明，掺杂稀bicelle解决方案与少量的带电两亲物大大提高了稳定性和程度的排列，以及扩展这些系统的温度范围内的取向。根据胶体的DLVO理论解释了双胞聚集对样品组成的依赖性。
Dissolving biological macromolecules in dilute bicelle solutions, which form oriented liquid crystals in the presence of a magnetic field, permits measurement of anisotropic spin interactions such as dipolar couplings [Tjandra, N. and Bax, A., Science, 278, 1111-1114]. However, the Lifetimes and temperature ranges of orientation for these samples are critically dependent on sample composition and experimental conditions. This paper demonstrates that doping dilute bicelle solutions with small amounts of charged amphiphiles substantially improves the stability and degree of alignment, as well as extends the temperature range of orientation for these systems. An explanation of the dependence of bicelle aggregation on sample composition is proposed based on the DLVO theory of colloids.