Molecular cloning of Mu d(bla lacZ) transcriptional and translational fusions.
Molecular cloning of Mu d(bla lacZ) transcriptional and translational fusions.
复制标题
Mu d(bla lacZ) 转录和翻译融合体的分子克隆。
DOI：
10.1128/jb.169.5.2026-2030.1987
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发表时间：
1987
影响因子：
3.2
通讯作者：
Wanner,BL
中科院分区：
文献类型：
--
作者：
Wanner,BL
载体pBW 2被制成选择性地克隆具有染色体Mu d（bla lacZ）转录或翻译融合的嵌合质粒。它是四环素耐药的，其PstI位点远端的bla的羧基末端。因为连接PstI消化的染色体DNA的Mu d（bla lacZ）插入与pBW 2恢复bla，氨苄青霉素抗性嵌合质粒是可选择的。这些质粒具有Mu d bla氨基末端，同时获得其他Mu d序列，包括lacZ、染色体融合接头和邻近最近的染色体Pst I位点的DNA。质粒pBW 2可用于几个psi和pho：：lacZ（Mu d）融合体以及位于Mu d插入附近的染色体基因的分子克隆。
The vector pBW2 was made to selectively clone chimeric plasmids with chromosomal Mu d(bla lacZ) transcriptional or translational fusions. It was tetracycline resistant and had the carboxyl-terminal end of bla distal to its PstI site. Because ligation of PstI-digested chromosomal DNA of a Mu d(bla lacZ) insertion with pBW2 restored bla, ampicillin-resistant chimeric plasmids were selectable. These plasmids had the Mu d bla amino terminus and simultaneously acquired other Mu d sequences including lacZ, the chromosomal fusion joint, and the DNA adjacent to the nearest chromosomal PstI site. The plasmid pBW2 was useful in the molecular cloning of several psi and pho::lacZ(Mu d) fusions, as well as chromosomal genes located near Mu d insertions.