Complexes of star-shaped cationic polyelectrolytes with anionic liposomes: Towards multi-liposomal assemblies with controllable stability
Complexes of star-shaped cationic polyelectrolytes with anionic liposomes: Towards multi-liposomal assemblies with controllable stability
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DOI：
10.1016/j.polymer.2016.04.025
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发表时间：
2016-06
期刊：
影响因子：
4.6
通讯作者：
A. Sybachin;O. V. Zaborova;D. Pergushov;A. Zezin;F. Plamper;A. Müller;E. Kesselman;Judith Schmidt;Y. Talmon;F. Menger;A. Yaroslavov
中科院分区：
文献类型：
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作者：
A. Sybachin;O. V. Zaborova;D. Pergushov;A. Zezin;F. Plamper;A. Müller;E. Kesselman;Judith Schmidt;Y. Talmon;F. Menger;A. Yaroslavov
通过30-50 nm阴离子脂质体和星形聚{[2-（甲基丙烯酰氧基）乙基]三甲基碘化铵}之间的静电相互作用形成复合物，所述星形聚{[2-（甲基丙烯酰氧基）乙基]三甲基碘化铵}具有从硅基中心核辐射的阳离子臂。研究络合作用，注意评估阳离子星状物对于阴离子脂质体（液体和固体）的能力;络合脂质体的完整性;和所得星星/脂质体络合物在盐水溶液中的稳定性。我们发现通过改变脂质体膜上阴离子基团的含量以及膜的相态，可以控制星星/脂质体复合物在盐水介质中的稳定性。含有0.1摩尔分数的阴离子棕榈酰油酰磷脂酰丝氨酸（POPS 1-）的液体脂质体和含有0.1摩尔分数的POPS 1-的固体脂质体在与星结合时保持其完整性，所得的星星/脂质体复合物在生理溶液（即[NaCl] = 0.15 M）中稳定。每星星含有多达12个脂质体的多脂质体复合物似乎有望作为生物活性化合物的载体。
Complexes were formed via the electrostatic interaction between 30–50 nm anionic liposomes and a star-shaped polyelectrolyte, poly{[2-(methacryloyloxy)ethyl]trimethyl ammonium iodide}, having cationic arms that radiate from a silicon-based central core. The complexation was investigated with attention given to assessing the capacity of the cationic stars for the anionic liposomes (both liquid and solid); the integrity of the complexed liposomes; and the stability of the resulting star/liposome complexes in aqueous salt solutions. We have found that by changing the content of anionic groups in the liposomal membrane as well as the phase-state of membrane, the stability of star/liposome complexes in aqueous salt media can be controlled. The liquid liposomes with 0.1 mol fraction of anionic palmitoyloleoylphosphatidylserine (POPS1−), and solid liposomes with 0.1 mol fraction of POPS1−, retain their integrity when bound to the stars, with the resulting star/liposome complexes being stable in physiological solution, i.e. [NaCl] = 0.15 M. Multi-liposomal complexes containing up to 12 liposomes per star seem to hold promise as carriers for biologically active compounds.