Transcriptional cascades during spermatogenesis: pivotal role of CREM and ACT
Transcriptional cascades during spermatogenesis: pivotal role of CREM and ACT
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DOI：
10.1016/s0303-7207(01)00463-4
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发表时间：
2001-06-20
影响因子：
4.1
通讯作者：
Sassone-Corsi, P
中科院分区：
文献类型：
--
作者：
Fimia, GM;Morlon, A;Sassone-Corsi, P
CREM基因在下丘脑-性腺轴中起着重要的生理和发育作用。我们先前已经证明CREM在雄性减数分裂后细胞中高表达。精子发生是一个复杂的过程，减数分裂后的麦芽：生殖细胞分化为成熟的精子。CREM调控一些参与精子发生过程的减数分裂后基因的表达。通过同源重组，我们获得了CREM突变小鼠，它们在精子发生的第一步表现出完整的阻断，CREM诱导其调节功能的分子机制涉及ACT(睾丸CREM激活因子)，这是一种由四个半LIM结构域重复组成的睾丸特异性共激活因子。ACT与CREM协同表达。与之相关联，并赋予其强大的转录激活功能。它可以绕过经典的CREM磷酸化和CBP募集的要求。(C)2001爱思唯尔爱尔兰科学有限公司。保留所有权利。
The gene CREM plays key physiological and developmental roles within the hypothalamic pituitary-gonadal axis. We have previously shown that CREM is highly expressed in male postmeiotic cells. Spermiogenesis is a complex process by which postmeiotic malt: germ cells differentiate into mature spermatozoa. CREM regulates the expression of a number of post-meiotic genes involved in the process of spermiogenesis. Using homologous recombination we have generated CREM-mutant mice that display a complete block at the first step of spermiogenesis, The molecular mechanism by which CREM elicits its regulatory function involves ACT (Activator of CREM in Testis), a testis-specific coactivator constituted by a repeat of four and half LIM domains. ACT is coordinately expressed with CREM. associates with it and confers a powerful transcriptional activation function. It is able to bypass the classical requirement of CREM phosphorylation and recruiting of CBP. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.