INTERACTIONS OF CELLULAR PROTEINS INVOLVED IN THE TRANSCRIPTIONAL REGULATION OF THE HUMAN-IMMUNODEFICIENCY-VIRUS
INTERACTIONS OF CELLULAR PROTEINS INVOLVED IN THE TRANSCRIPTIONAL REGULATION OF THE HUMAN-IMMUNODEFICIENCY-VIRUS
复制标题
DOI：
10.1002/j.1460-2075.1987.tb02711.x
复制
发表时间：
1987-12-01
期刊：
影响因子：
11.4
通讯作者：
GAYNOR, RB
中科院分区：
文献类型：
--
作者：
GARCIA, JA;WU, FK;GAYNOR, RB
人类免疫缺陷病毒（HIV）是一种人类逆转录病毒，是获得性免疫缺陷综合症的病原体。为了研究参与该病毒转录调控的细胞因子，我们使用部分纯化的HeLa细胞提取物对病毒LTR进行了DNA酶I足迹法。病毒LTR的五个区域对于细胞蛋白的DNA结合似乎是关键的。这些包括负调控区、增强子区、SP1区、TATA区和非翻译区。这些结合结构域的缺失诱变对转录的基础水平和被病毒达特蛋白诱导的能力具有显著影响。负调控区或非翻译区的突变影响因子与增强子区的结合。此外，与几个结合结构域互补的寡核苷酸特异性竞争因子结合。这些结果表明，几种不同的细胞蛋白质之间的相互作用是HIV转录调控所必需的。
The human immunodeficiency virus (HIV) is a human retrovirus which is the etiologic agent of the acquired immunodeficiency syndrome. To study the cellular factors involved in the transcriptional regulation of this virus, we performed DNase I footprinting of the viral LTR using partially purified HeLa cell extracts. Five regions of the viral LTR appear critical for DNA binding of cellular proteins. These include the negative regulatory, enhancer, SP1, TATA and untranslated regions. Deletion mutagenesis of these binding domains has significant effects on the basal level of transcription and the ability to be induced by the viral tat protein. Mutations of either the negative regulatory or untranslated regions affect factor binding to the enhancer region. In addition, oligonucleotides complementary to several of the binding domains specifically compete for factor binding. These results suggest that interactions between several distinct cellular proteins are required for HIV transcriptional regulation.