An ultra-sensitive ratiometric fluorescent probe for hypochlorous acid detection by the synergistic effect of AIE and TBET and its application of detecting exogenous/endogenous HOCl in living cells
An ultra-sensitive ratiometric fluorescent probe for hypochlorous acid detection by the synergistic effect of AIE and TBET and its application of detecting exogenous/endogenous HOCl in living cells
复制标题
AIE和TBET协同作用检测次氯酸的超灵敏比率荧光探针及其在检测活细胞中外源/内源HOCl中的应用
DOI：
10.1039/c9tb01279f
复制
发表时间：
2019-09-07
影响因子：
7
通讯作者：
Sun, Hongyan
中科院分区：
文献类型：
--
作者：
Duan, Qinya;Zheng, Guansheng;Sun, Hongyan
本文报道了一种基于聚集诱导发射（AIE）和键合能量转移（TBET）机制的用于次氯酸（HOCl）检测的超灵敏比率荧光探针。通过利用 AIE 和 TBET 的优势，消除暗供体的发射泄漏，该探针对 HOCl 表现出超高灵敏度，荧光比 (I-589 nm/I-477 nm) 提高了 7000 倍以上，是迄今为止记录的最高水平之一。详细讨论了反应机理，并研究了干扰物的影响和反应动力学。最后，在不同细胞系中外源/内源 HOCl 成像检测的成功结果表明该探针在生命系统中的潜在用途。
An ultra-sensitive and ratiometric fluorescent probe for hypochlorous acid (HOCl) detection based on the mechanism of aggregation induced emission (AIE) and through-bond energy transfer (TBET) has been reported herein. By exploiting the advantages of AIE and TBET, which eliminates emission leakage from dark donors, the probe exhibits ultra-high sensitivity towards HOCl by an enhancement of over 7000-fold in the fluorescence ratio (I-589 nm/I-477 nm), which is one of the highest recorded so far. The reaction mechanism has been discussed in detail, and the effects of interferents and the reaction kinetics have also been investigated. Lastly, the successful result of exogenous/endogenous HOCl imaging detection in different cell lines indicates the potential use of the probe in living systems.