The chromosome partitioning protein, ParB, is required for cytokinesis in Caulobacter crescentus
The chromosome partitioning protein, ParB, is required for cytokinesis in Caulobacter crescentus
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DOI：
10.1046/j.1365-2958.2001.02643.x
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发表时间：
2001-11-01
影响因子：
3.6
通讯作者：
Gober, JW
中科院分区：
文献类型：
--
作者：
Mohl, DA;Easter, J;Gober, JW
在新月柄杆菌（Caulobacter crescentus）中，编码染色体分离蛋白ParA和ParB的基因是必需的。ParB的缺失会导致细胞呈光滑丝状，且DNA复制仍在继续。免疫荧光显微镜观察显示，在缺乏ParB的细胞中，位于细胞中部的FtsZ环的形成受到了阻碍，而FtsZ环的形成是细菌细胞分裂起始过程中最早的分子事件。ParB结合在新月柄杆菌复制起点附近的序列上。细胞周期实验表明，两极定位的ParB焦点的形成，以及推测的复制起点在细胞两极的定位，比细胞中部FtsZ环的形成早20分钟。这些结果表明，ParA和ParB的一个主要功能可能是调节新月柄杆菌胞质分裂的起始。
In Caulobacter crescentus, the genes encoding the chromosome partitioning proteins, ParA and ParB, are essential. Depletion of ParB resulted in smooth filamentous cells in which DNA replication continued. Immunofluorescence microscopy revealed that the formation of FtsZ rings at the mid-cell, the earliest molecular event in the initiation of bacterial cell division, was blocked in cells lacking ParB. ParB binds sequences near the C. crescentus origin of replication. Cell cycle experiments show that the formation of bipolarly localized ParB foci, and presumably localization of the origin of replication to the cell poles, preceded the formation of FtsZ rings at the mid-cell by 20 min. These results suggest that one major function of ParA and ParB may be to regulate the initiation of cytokinesis in C. crescentus.