Translocation and rearrangement of myeloperoxidase gene in acute promyelocytic leukemia.
Translocation and rearrangement of myeloperoxidase gene in acute promyelocytic leukemia.
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急性早幼粒细胞白血病中髓过氧化物酶基因的易位和重排。
DOI：
10.1126/science.2896388
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
LeBeau,MM
中科院分区：
文献类型：
--
作者：
Weil,SC;Rosner,GL;Reid,MS;Chisholm,RL;Lemons,RS;Swanson,MS;Carrino,JJ;Diaz,MO;LeBeau,MM
急性早幼粒细胞白血病（M3亚型）的特征是恶性早幼粒细胞表现出大量异常大或异常的初级颗粒。髓过氧化物酶（MPO）活性的这些嗜天青颗粒，作为评估的细胞化学染色，是异常强烈。此外，M3普遍与染色体易位t（15;17）（q22;q11.2）相关。本研究通过体细胞杂交和染色体原位杂交，将MPO基因定位于人17号染色体（q12-q21）上，即t（15;17）的断裂点区域。通过MPO互补DNA克隆的原位杂交和Southern印迹分析，研究了这种特异性易位对MPO基因的影响。在所有检查的M3病例中，MPO易位至15号染色体。基因组印迹分析表明2/4例白血病细胞MPO重排。这些结果提示MPO在急性早幼粒细胞白血病的发病机制中可能起关键作用。
Acute promyelocytic leukemia (subtype M3) is characterized by malignant promyelocytes exhibiting an abundance of abnormally large or aberrant primary granules. Myeloperoxidase (MPO) activity of these azurophilic granules, as assessed by cytochemical staining, is unusually intense. In addition, M3 is universally associated with a chromosomal translocation, t(15;17)(q22;q11.2). In this report, theMPOgene was localized to human chromosome 17 (q12-q21), the region of the breakpoint on chromosome 17 in the t(15;17), by somatic cell hybrid analysis and in situ chromosomal hybridization. By means ofMPOcomplementary DNA clones for in situ hybridization and Southern blot analysis, the effect of this specific translocation on theMPOgene was examined. In all cases of M3 examined,MPOis translocated to chromosome 15. Genomic blot analyses indicate rearrangement ofMPOin leukemia cells of two of four cases examined. These findings suggest thatMPOmay be pivotal in the pathogenesis of acute promyelocytic leukemia.