MOLECULAR EVIDENCE THAT INSECTICIDE RESISTANCE IN PEACH POTATO APHIDS (MYZUS-PERSICAE SULZ) RESULTS FROM AMPLIFICATION OF AN ESTERASE GENE
MOLECULAR EVIDENCE THAT INSECTICIDE RESISTANCE IN PEACH POTATO APHIDS (MYZUS-PERSICAE SULZ) RESULTS FROM AMPLIFICATION OF AN ESTERASE GENE
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DOI：
10.1042/bj2510309
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发表时间：
1988-04-01
影响因子：
4.1
通讯作者：
FORDE, BG
中科院分区：
文献类型：
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作者：
FIELD, LM;DEVONSHIRE, AL;FORDE, BG
桃-马铃薯蚜虫（Myzus persicae苏尔茨）对杀虫剂的广泛抗性的酯酶（E4）的cDNA克隆分离并用于研究抗性的分子基础。抗性蚜虫酯酶合成的增加与酯酶结构基因（E4或其密切相关的变体，FE 4）的扩增有关，扩增的程度与酯酶的活性和抗性水平相关。杂交的cDNA克隆基因组Southern印迹显示，只有一些酯酶相关的限制性片段扩增。抗性蚜虫不同克隆的限制性图谱之间的质的差异与特定染色体易位的存在或不存在以及E4或FE 4的产生相关。
cDNA clones for the esterase (E4) responsible for broad insecticide resistance in peach-potato aphids (Myzus persicae Sulz.) were isolated and used to study the molecular basis of resistance. Increased esterase synthesis by resistant aphids was found to be associated with amplification of the structural gene for the esterase (E4 or its closely related variant, FE4), the degree of amplification being correlated with the activity of the esterase and the level of resistance. Hybridization of the cDNA clones to genomic Southern blots showed that only some of the esterase-related restriction fragments are amplified. Qualitative differences between restriction patterns in different clones of resistant aphids correlated with the presence or absence of a specific chromosome translocation and with production of E4 or FE4.