Lipid transport mechanism in the fish-V. Isolation and characterization of carp plasma lipoproteins.
Lipid transport mechanism in the fish-V. Isolation and characterization of carp plasma lipoproteins.
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鱼中的脂质转运机制-V．
DOI：
10.2331/suisan.56.1113
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发表时间：
1990
影响因子：
0.4
通讯作者：
M. Kayama
中科院分区：
文献类型：
--
作者：
N. Iijima;T. Ueki;S. Yoshitake;M. Kayama
通过连续超速离心法从饲喂后 8 小时获得的鲤鱼血浆中分离 VLDL（d<1.006g/ml）、LDL1（d 1.006-1.063g/ml）、LDL2（d 1.063-1.125g/ml）和 HDL（d 1.125-1.21g/ml），并通过凝胶过滤柱色谱纯化。鲤鱼 VLDL、LDL2 和 HDL 均检测为单条带，但 LDL1 通过聚丙烯酰胺凝胶电泳进行脂质染色，由 3 个条带组成。 VLDL和LDL1的主要脂质成分是甘油三酯。此外，VLDL 和 LDL1 均含有人类载脂蛋白 B、载脂蛋白 A-II 和载脂蛋白 C 样蛋白。除甘油三酯外，鲤鱼 LDL2 还含有相对较高水平的甾醇酯，并且仅含有人类载脂蛋白 B 样蛋白。鲤鱼HDL是所有脂蛋白中的主要类别，含有磷脂作为主要脂质成分和人载脂蛋白A-I-和A-II样蛋白作为主要脱辅基蛋白成分。
VLDL (d<1.006g/ml), LDL1(d 1.006-1.063g/ml), LDL2 (d 1.063-1.125g/ml), and HDL (d 1.125-1.21g/ml) were isolated by sequential ultracentrifugation from carp plasma obtained at 8h after feeding and purified by gel-filtration column chromatographies. Carp VLDL, LDL2, and HDL were all detected as a single band, but LDL1 consisted of three bands with lipid staining by polyacrylamide gel electrophoresis. The major lipid components of VLDL and LDL1 were triglycerides. Moreover, both VLDL and LDL1 contained human apo B-, apo A-II-, and apo C- like proteins. Carp LDL2 contained relatively high levels of sterol esters in addition to trig-lycerides, and contained exclusively human apo B-like protein. Carp HDL, which was the pre-dominant class in all lipoproteins, contained phospholipids as the major lipid components and human apo A-I-and A-II-like proteins as the major apoprotein components.