TAF7 is a heat-inducible unstable protein and is required for sustained expression of heat shock protein genes
TAF7 is a heat-inducible unstable protein and is required for sustained expression of heat shock protein genes
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TAF7 是一种热诱导不稳定蛋白，是热休克蛋白基因持续表达所必需的
DOI：
10.1111/febs.14604
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Sakurai Hiroshi
中科院分区：
文献类型：
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作者：
Nagashimada Mayumi;Ueda Takumi;Ishita Yuichiro;Sakurai Hiroshi
TATA结合蛋白相关因子7（TAF 7）是一般转录因子IID（TFIID）的可解离组分，在RNA聚合酶II（Pol II）转录起始步骤中起着检查点调节剂的作用。在这里，我们专注于TAF 7在热休克细胞中的作用，其表达由热休克因子HSF 1诱导。TAF 7是一种磷蛋白，其磷酸化状态与其与TFIID的相互作用及其由泛素-蛋白酶体途径控制的稳定性有关。TAF 7对于热休克蛋白基因的延长表达和热休克细胞的有效恢复是必需的。在持续转录过程中，TAF 7（可能是其TFIID非依赖性形式）结合启动子并增强基因体处的Pol II水平，但不增强启动子。这些结果显示了TAF 7的新功能，其对于多轮转录中从起始到延伸的转变是必需的。
TATA‐binding protein‐associated factor 7 (TAF7), a dissociable component of the general transcription factor IID (TFIID), plays a role as a check‐point regulator at the step of RNA polymerase II (Pol II) transcription initiation. Here, we focused on the role of TAF7 in heat‐shocked cells, where its expression is induced by heat shock factor HSF1. TAF7 is a phosphoprotein, and the phosphorylation status is related to its interaction with TFIID and to its stability controlled by the ubiquitin‐proteasome pathway. TAF7 is necessary for the prolonged expression of heat shock protein genes and for efficient recovery of heat‐shocked cells. During sustained transcription, TAF7, presumably its TFIID‐independent form, binds the promoter and enhances the levels of Pol II at the gene body but not the promoter. These results showed the novel function of TAF7 that is necessary for the transition from initiation to elongation in multiple‐round transcription.