Production of PHB by a Bacillus megaterium strain using sugarcane molasses and corn steep liquor as sole carbon and nitrogen sources
Production of PHB by a Bacillus megaterium strain using sugarcane molasses and corn steep liquor as sole carbon and nitrogen sources
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DOI：
10.1078/0944-5013-00104
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发表时间：
2001-01-01
影响因子：
6.7
通讯作者：
Omar, SH
中科院分区：
文献类型：
--
作者：
Gouda, MK;Swellam, AE;Omar, SH
聚羟基丁酸（PHB）和其他生物降解聚酯是开发环境友好的、完全生物降解塑料的有希望的候选者。甘蔗糖蜜和玉米浆是埃及最便宜的两种底物，使用这两种底物可能有助于降低生产这种生物聚酯的成本。本文研究了不同碳源的影响。以甘蔗糖蜜和葡萄糖为唯一碳源时，PHB的产量最高，分别为40.8、39.9/mg细胞干物质。糖蜜浓度为3%时生长最好，而2%时细胞干物质中聚羟基丁酸的产量最高（46.2%）。玉米浆是最佳的氮源（32.7mg/细胞干物质），而氯化铵、硫酸铵、草酸铵和磷酸铵则是最佳的氮源。
Poly(hydroxybutyric acid) (PHB) and other biodegradable polyesters are promising candidates for the development of environment-friendly, totally biodegradable plastics. The use of cane molasses and corn steep liquor, two of the cheapest substrates available in Egypt, may help to reduce the cost of producing such biopolyesters. In this work, the effect of different carbon sources was studied. Maximum production of PHB was obtained with cane molasses and glucose as sole carbon sources (40.8, 39.9 per mg cell dry matter, respectively). The best growth was obtained with 3% molasses, while maximum yield of PHB (46.2% per mg cell dry matter) was obtained with 2% molasses. Corn steep liquor was the best nitrogen source for PHB synthesis (32.7 mg per cell dry matter), on the other hand, best growth was observed when ammonium chloride, ammonium sulphate, ammonium oxalate or ammonium phosphate were used as nitrogen sources.