Effects of pH, ionic strength, and solutes on DNA adsorption by andosols
Effects of pH, ionic strength, and solutes on DNA adsorption by andosols
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DOI：
10.1007/s00374-010-0447-y
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发表时间：
2010-02
影响因子：
6.5
通讯作者：
K. Saeki;T. Kunito;M. Sakai
中科院分区：
文献类型：
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作者：
K. Saeki;T. Kunito;M. Sakai
研究了两种 Andosol 对 DNA 的吸附随溶液 pH 值、溶液中离子强度和某些溶质的变化，以了解细胞外 DNA 分子在两种 Andosol 中的行为。在 3.0-9.0 的 pH 范围内，通过增加悬浮液的 pH 值，DNA 吸附大大减少。两种安多溶胶对 DNA 分子的吸附不受 0.1 至 0.5 mol L−1NaCl 离子强度的影响。然而，DNA 吸附量随着悬浮液中 Mg2+ 浓度的增加而成比例增加。磷酸盐的添加降低了 DNA 吸附，表明 DNA 分子和磷酸根离子在安土溶胶的吸附中存在竞争。这些结果表明土壤中存在多种 DNA 吸附机制。
The adsorption of DNA by two andosols was investigated as function of solution pH, ionic strength in solution and some solutes, so as to understand the behavior of extracellular DNA molecules in two andosols. DNA adsorptions greatly decreased by increasing pH of suspensions in a pH range of 3.0–9.0. The adsorption of DNA molecules by both andosols was not affected by ionic strength from 0.1 to 0.5 mol L−1NaCl. However, the DNA adsorption increased proportionally by increasing Mg2+concentration in the suspension. Addition of phosphate decreased DNA adsorption, indicating competition between DNA molecules and phosphate ion in the adsorption by andosols. These results suggest that there are several DNA adsorption mechanisms in soil.