Elucidation of the control mechanism of dynamic tissues using freezing techniques
Elucidation of the control mechanism of dynamic tissues using freezing techniques
复制标题
使用冷冻技术阐明动态组织的控制机制
DOI：
10.1007/s12565-022-00648-9
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发表时间：
2022
影响因子：
1.2
通讯作者：
Yurika Saitoh
中科院分区：
文献类型：
--
作者：
Tsukamoto S;Errani C;Kido A;Mavrogenis AF.;Yurika Saitoh
动物和人类组织学标本的制备是一个多步骤的过程，包括组织收集、固定和脱水，然后进行石蜡包埋。每个过程可以使用不同的方法和物质来实现。例如，根据用于包埋的物质，可能不需要脱水。本研究中描述的冷冻技术可用于组织收集和固定。使用“体内冷冻技术（IVCT）”获得的组织反映了组织采集时体液中的血流和蛋白质定位，使其成为未来组织学分析中不可或缺的方法。本研究利用IVCT从多个角度捕获动态物体的组织学图像，并在分子水平上阐明其运动控制的机制。
The preparation of histological specimens from animals and humans is a multi-step process comprising tissue collection, fixation, and dehydration, followed by paraffin embedding. Each process can be achieved using different methods and substances. For example, dehydration may not be required depending on the substance used for embedding. The freezing technique described in the present study can be used for tissue collection and fixation. Tissues obtained using “in vivo cryotechnique (IVCT)” reflect blood flow and protein localization in body fluids at the time of tissue collection, making it an indispensable method in histological analyses of the future. This study utilized the IVCT to capture histological images of dynamic objects from multiple viewpoints and elucidate the mechanism underlying their movement control at the molecular level.