Useful immunohistochemical panel for differentiating clear cell papillary renal cell carcinoma from its mimics
Useful immunohistochemical panel for differentiating clear cell papillary renal cell carcinoma from its mimics
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DOI：
10.1016/j.anndiagpath.2013.05.004
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发表时间：
2013-10-01
影响因子：
2
通讯作者：
Bing, Zhanyong
中科院分区：
文献类型：
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作者：
Pramick, Michelle;Ziober, Amy;Bing, Zhanyong
透明细胞乳头状肾细胞癌（CCPRCC）是最近描述的一种低级别肾细胞肿瘤。在本研究中，我们通过免疫组织化学方法研究了 20 例 CCPRCC 肿瘤中配对盒 8 (PAX-8)、碳酸酐酶 IX (CA IX)、CK7 和 α-甲基酰基辅酶 A 消旋酶 (AMACR) 的表达。透明细胞乳头状肾细胞癌在每种情况下均显示 PAX-8 弥漫性 (70%) 或中等 (30%) 核阳性，主要为中等强度 (50%)。 90% 的病例显示一定程度的 CA IX 细胞质染色，主要为中等强度 (50%)。此外，每个CCPRCC病例还显示出弥漫性CK7膜染色。大多数 CCPRCC (95%) 的 AMACR 呈阴性。 PAX-8、CA IX、CK7 和 AMACR 组成了一个简洁的面板，用于区分 CCPRCC 与其模拟物。 PAX-8 阳性有助于确认该肿瘤的肾脏起源。 CA IX 和 CK7 阳性可将 CCPRCC 与传统透明细胞肾细胞癌区分开来，后者通常为 CA IX 阳性而 CK7 阴性。 CCPRCC 中的 CK7 阳性和 AMACR 阴性模式将其与乳头状肾细胞癌区分开来，乳头状肾细胞癌通常 AMACR 和 CK7 均呈阳性。 (C) 2013 Elsevier Inc. 保留所有权利。
Clear cell papillary renal cell carcinoma (CCPRCC) is a recently described low-grade renal cell tumor. In this study, we investigated the expression of paired box 8 (PAX-8), carbonic anhydrase IX (CA IX), CK7, and alpha-methylacyl-CoA-racemase (AMACR) in this tumor by immunohistochemistry in a group of 20 cases of CCPRCC. Clear cell papillary renal cell carcinoma showed diffuse (70%) or intermediate (30%) nuclear positivity for PAX-8 in each case, with predominantly moderate intensity (50%). Ninety percent of the cases showed some degree of cytoplasmic staining for CA IX, predominantly with moderate intensity (50%). In addition, each case of CCPRCC also showed diffuse membranous staining for CK7. Most CCPRCCs (95%) were negative for AMACR. PAX-8, CA IX, CK7, and AMACR comprise a concise panel for distinguishing CCPRCC from its mimics. PAX-8 positivity helps to confirm the renal origin of this tumor. Positivity for CA IX and CK7 differentiate CCPRCC from conventional clear cell renal cell carcinoma, which is usually CA IX positive while CK7 negative. The CK7-positive and AMACR-negative pattern seen in CCPRCC differentiates it from papillary renal cell carcinoma, which is usually positive for both AMACR and CK7. (C) 2013 Elsevier Inc. All rights reserved.