Kinetic modeling and isotopic investigation of isobutanol fermentation by two engineered escherichia coli strains
Kinetic modeling and isotopic investigation of isobutanol fermentation by two engineered escherichia coli strains
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两种工程大肠杆菌菌株异丁醇发酵的动力学模型和同位素研究
DOI：
10.1021/ie202936t
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发表时间：
2012
影响因子：
4.2
通讯作者：
Yinjie J. Tang
中科院分区：
文献类型：
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作者：
Yi Xiao;Xueyang Feng;Arul M. Varman;Lian He;Huifeng Yu;Yinjie J. Tang
我们构建了一株具有Ehrlich途径的大肠杆菌BL21菌株(生产异丁醇的低性能菌株)。我们还从加州大学的James Liao组获得了一株高产异丁醇的大肠杆菌JCL260。为了比较两株工程菌的发酵性能，我们采用了基于Monod的通用模型，并结合混合生长相关的异丁醇形成动力学来模拟不同培养条件下的葡萄糖消耗、生物量增长和产物分泌/损失。根据动力学数据和额外的13C同位素研究，我们发现该低性能菌株在最低限度的生长介质(20g/L葡萄糖)中表现出强劲的生物量增长，异丁醇产量(高达0.95g/L)。该菌在富营养液中生长时，利用大量酵母抽提物合成异丁醇。丰富的培养基还促进了废物的分泌，从而降低了葡萄糖基异丁醇的产率。相比之下..。
We constructed an Escherichia coli BL21 strain with the Ehrlich pathway (the low-performance strain for isobutanol production). We also obtained a high isobutanol-producing E. coli strain JCL260 from the James Liao group (University of California). To compare the fermentation performances of the two engineered strains, we employed a general Monod-based model coupled with mixed-growth-associated isobutanol formation kinetics to simulate glucose consumption, biomass growth, and product secretion/loss under different cultivation conditions. On the basis of both kinetic data and additional 13C-isotopic investigation, we found that the low-performance strain demonstrated robust biomass growth in the minimal growth medium (20 g/L glucose), achieving isobutanol production (up to 0.95 g/L). It utilized significant amounts of yeast extract to synthesize isobutanol when it grew in the rich medium. The rich medium also enhanced waste product secretion, and thus reduced the glucose-based isobutanol yield. In contrast...