Immunohistological Observation of Milled Teeth in a Rat Mandibular Incisor Extraction Socket
Immunohistological Observation of Milled Teeth in a Rat Mandibular Incisor Extraction Socket
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DOI：
10.2485/jhtb.23.29
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发表时间：
2014-01-01
影响因子：
0.4
通讯作者：
Tanaka, Yoshinobu
中科院分区：
文献类型：
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作者：
Matsukawa, Ryohei;Ozawa, Shogo;Tanaka, Yoshinobu
回收不需要的牙齿用作骨移植材料应该是增强牙槽骨缺损的骨形成过程并降低材料成本的一种有价值的方法。然而，使用移植材料形成骨的过程尚不完全清楚。本研究的目的是通过大鼠切牙拔除模型中的免疫组织学观察来评估包括铣削牙齿在内的材料的命运。将冷冻研磨的切牙材料注入大鼠下颌骨的拔牙槽中。术后4周取下颌骨标本，采用贴膜法制备冰冻切片，免疫组化染色观察DMP-1和CD68。结果显示，术后四个星期，磨碎的牙齿颗粒残留在牙槽周围，并且根据颗粒大小表现出不同的反应。小颗粒（3-10μm）被CD68阳性细胞吞噬，而骨再生发生在大颗粒（30-60μm）周围。这些结果表明，注射碎牙可以为拔牙槽中的骨形成提供最佳的支架和生长因子。
Recycling unnecessary teeth for use as a bone grafting material should be a valuable approach to enhance the bone-forming process in alveolar bone defects and to reduce material costs. However, the process of bone formation using grafting material is not fully understood. The aim of this study was to assess the fate of material including milled teeth by immunohistological observation in a rat incisor extraction model. Freeze-milled incisor material was injected into an extraction socket in a rat mandible bone. Four weeks after the operation, the mandible was sampled and frozen sections were prepared by the sticking film method, after which DMP-1 and CD68 were visualized by immunohistochemical staining. The results revealed that milled tooth particles remained around the socket at four weeks post-operation, and showed different reactions depending on the particle size. Small particles (3-10 mu m) were phagocytosed by CD68-positive cells, whereas bone regeneration occurred around large (30-60 mu m) particles. These results suggest that the injection of crushed teeth could provide an optimum scaffold and growth factors for bone formation in extraction sockets.