KIF1B, A NOVEL MICROTUBULE PLUS END-DIRECTED MONOMERIC MOTOR PROTEIN FOR TRANSPORT OF MITOCHONDRIA
KIF1B, A NOVEL MICROTUBULE PLUS END-DIRECTED MONOMERIC MOTOR PROTEIN FOR TRANSPORT OF MITOCHONDRIA
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DOI：
10.1016/0092-8674(94)90012-4
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发表时间：
1994-12-30
期刊：
影响因子：
64.5
通讯作者：
HIROKAWA, N
中科院分区：
文献类型：
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作者：
NANGAKU, M;SATOYOSHITAKE, R;HIROKAWA, N
为了进一步阐明细胞器运输的机制，我们克隆了小鼠驱动蛋白超家族的一个成员KIF 1B。这种N-末端型马达蛋白在各种组织中普遍表达。原位杂交显示KIF 1B在分化的神经细胞中大量表达。有趣的是，KIF 1B作为单体发挥作用，具有微管和末端定向运动性，我们的旋转阴影电子显微镜显示大多是单个球状结构。免疫细胞化学，KIF 1B与线粒体在体内共定位。此外，亚细胞分级分离研究表明，KIF 1B集中在线粒体组分，纯化的KIF 1B可以在体外沿沿着微管运输线粒体。这些数据有力地表明，KIF 1B作为单体马达进行线粒体的顺行运输。
To further elucidate the mechanism of organelle transport, we cloned a navel member of the mouse kinesin superfamily, KIF1B. This N-terminal-type motor protein is expressed ubiquitously in various kinds of tissues. In situ hybridization revealed that KIF1B is expressed abundantly in differentiated nerve cells. Interestingly, KIF1B works as a monomer, having a microtubule plus end-directed motility, Our rotary shadowing electron microscopy revealed mostly single globular structures. Immunocytochemically, KIF1B was colocalized with mitochondria in vivo. Furthermore, a subcellular fractionation study showed that KIF1B was concentrated in the mitochondrial fraction, and purified KIF1B could transport mitochondria along microtubules in vitro. These data strongly suggested that KIF1B works as a monomeric motor for anterograde transport of mitochondria.