Oligonucleotide microchips as genosensors for determinative and environmental studies in microbiology
Oligonucleotide microchips as genosensors for determinative and environmental studies in microbiology
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DOI：
10.1128/aem.63.6.2397-2402.1997
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发表时间：
1997-06-01
影响因子：
4.4
通讯作者：
Mirzabekov, AD
中科院分区：
文献类型：
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作者：
Guschin, DY;Mobarry, BK;Mirzabekov, AD
对环境核酸与固定在载玻片上聚丙烯酰胺凝胶垫基质中的许多寡核苷酸（寡核苷酸微芯片）进行平行杂交的效用进行了评估。与选定微生物类群的小亚基核糖体RNA序列互补的寡核苷酸，包括硝化细菌的关键属，被证明能够选择性地保留来自目标序列的DNA或RNA形式的标记靶核酸。改变探针浓度以标准化杂交信号的效用以及使用多色检测对多个探针 - 靶标群体同时进行定量的方法得到了验证。
The utility of parallel hybridization of environmental nucleic acids to many oligonucleotides immobilized in a matrix of polyacrylamide gel pads on a glass slide (oligonucleotide microchip) was evaluated. Oligonucleotides complementary to small-subunit rRNA sequences of selected microbial groups, encompassing key genera of nitrifying bacteria, were shown to selectively retain labeled target nucleic acid derived from either DNA or RNA forms of the target sequences. The utility of varying the probe concentration to normalize hybridization signals and the use of multicolor detection for simultaneous quantitation of multiple probe-target populations were demonstrated.