Use of metabolic stimulators and inhibitors for enhanced production of β-carotene and lycopene by Blakeslea trispora NRRL 2895 and 2896
Use of metabolic stimulators and inhibitors for enhanced production of β-carotene and lycopene by Blakeslea trispora NRRL 2895 and 2896
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DOI：
10.1016/j.biortech.2007.05.051
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发表时间：
2008-05-01
影响因子：
11.4
通讯作者：
Singhal, Rekha S.
中科院分区：
文献类型：
--
作者：
Choudhari, Sheetal M.;Ananthanarayan, Laxmi;Singhal, Rekha S.
这项工作研究了代谢刺激物的潜力，首先是提高β -胡萝卜素的产生，然后使用番茄红素环化酶抑制剂，以便在发酵培养基中积累番茄红素。研究了各种非离子表面活性剂、天然油脂、刺激剂如氨基酸、三羧酸循环（TCA）中间体、维生素A和抗生素，以提高结合菌黑霉三孢菌的β -胡萝卜素产量。Span 20在0.2%时使β -胡萝卜素的产量从139 mg/l增加到318 mg/l。对跨度为20的三孢霉菌丝形态的检测表明，三孢霉菌丝长度较短，这使得三孢霉生长分散良好。在培养基中添加1000 ppm的维生素A醋酸盐，β -胡萝卜素的浓度最高（830 +/- 6 mg/l）。然后评估了咪唑、吡啶、三乙胺、胡椒碱和烟酸等几种化学抑制剂对番茄红素生物合成的阻断作用。500 ppm的哌啶提高了7.76倍，并在补充维生素a醋酸酯的培养基中产生了高滴度的番茄红素（775 5mg /l）。(c) 2007 Elsevier Ltd.版权所有。
This work investigates the potential of metabolic stimulators, firstly to enhance the production of beta-carotene, and later use of inhibitors of lycopene cyclase so as to accumulate lycopene in the fermentation medium. Various non-ionic surfactants, natural oils, stimulators such as amino-acids, tricarboxylic acid cycle (TCA) intermediates, vitamin A and antibiotics were investigated for improved production of beta-carotene using the zygomycete fungus Blakeslea trispora. Span 20 at 0.2% increased the beta-carotene production from 139 mg/l to 318 mg/l. Examination of the mycelial morphology of the R trispora with span 20 showed a shorter mycelial length, which allowed a well-dispersed growth of B. trispora. Supplementation of the medium with 1000 ppm vitamin A acetate gave highest concentration of beta-carotene (830 +/- 6 mg/l). Several chemical inhibitors such as imidazole, pyridine, triethylamine, piperidine, and nicotinic acid were then evaluated to block the biosynthesis at lycopene. Piperidine at 500 ppm gave a 7.76-fold improvement, and produced high titers of lycopene (775 5 mg/l) in a medium supplemented with vitamin A acetate. (c) 2007 Elsevier Ltd. All rights reserved.