Whole planarian chromosome squash.
Whole planarian chromosome squash.
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DOI：
10.1016/j.xpro.2020.100257
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发表时间：
2021-03-19
期刊：
影响因子：
--
通讯作者：
Oviedo NJ
中科院分区：
文献类型：
--
作者：
Barghouth PG;Oviedo NJ
整个涡虫染色体挤压使研究人员能够定性地分析染色体的完整性。秋水仙碱治疗用于中期停止细胞分裂，不需要截肢或穿刺组织。与醋酸-orcein（一种染色体固定剂）联合使用，该策略适用于由药物治疗、辐射和RNA干扰表型引起的组织脆弱的动物。这里介绍的整个涡虫挤压方法是一种微创程序，便于同时分析对照动物和实验动物的染色体完整性。有关使用和执行本协议的完整详情，请参阅。全涡虫染色体完整性分析全涡虫染色体完整性分析是微创的，不需要截肢或穿刺等程序，在由药物治疗或RNAi表型引起的脆弱组织中是可行的，对照和实验样品并行分析全涡虫染色体挤压使研究人员能够定性分析染色体完整性。秋水仙碱治疗用于中期停止细胞分裂，不需要截肢或穿刺组织。与醋酸-orcein（一种染色体固定剂）联合使用，该策略适用于由药物治疗、辐射和RNA干扰表型引起的组织脆弱的动物。这里介绍的整个涡虫挤压方法是一种微创程序，便于同时分析对照动物和实验动物的染色体完整性。
Whole planarian chromosome squash allows researchers to qualitatively analyze chromosome integrity. Treatment with colchicine is used to halt dividing cells within metaphase and does not require amputation or tissue puncturing. In combination with acetic-orcein, a stain-fixative for chromosomes, this strategy is suitable for animals with friable tissues caused by drug treatment, radiation, and RNA interference phenotypes. The whole planarian squash method presented here is a minimally invasive procedure that facilitates simultaneous analysis of chromosomal integrity in control and experimental animals. For complete details on the use and execution of this protocol, please refer to. Analysis of whole planarian chromosome integrity Minimally invasive and does not require procedures such as amputation or puncture Feasible in friable tissues resulting from pharmacological treatments or RNAi phenotypes Analysis in parallel of control and experimental samples Whole planarian chromosome squash allows researchers to qualitatively analyze chromosome integrity. Treatment with colchicine is used to halt dividing cells within metaphase and does not require amputation or tissue puncturing. In combination with acetic-orcein, a stain-fixative for chromosomes, this strategy is suitable for animals with friable tissues caused by drug treatment, radiation, and RNA interference phenotypes. The whole planarian squash method presented here is a minimally invasive procedure that facilitates simultaneous analysis of chromosomal integrity in control and experimental animals.
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影响因子：
5.4
作者：
Guo L;Accorsi A;He S;Guerrero-Hernández C;Sivagnanam S;McKinney S;Gibson M;Sánchez Alvarado A
通讯作者：
Sánchez Alvarado A
DOI：
10.1007/s00018-017-2697-4
发表时间：
2018-04
期刊：
Cellular and molecular life sciences : CMLS
影响因子：
--
作者：
Thiruvalluvan M;Barghouth PG;Tsur A;Broday L;Oviedo NJ
通讯作者：
Oviedo NJ
影响因子：
0.9
作者：
Knakievicz, Tanise;Lau, Adriana Helena;Erdtmann, Bernardo
通讯作者：
Erdtmann, Bernardo
影响因子：
7.3
作者：
Barghouth PG;Thiruvalluvan M;LeGro M;Oviedo NJ
通讯作者：
Oviedo NJ
影响因子：
4.6
作者：
Peiris, T. Harshani;Ramirez, Daniel;Oviedo, Nestor J.
通讯作者：
Oviedo, Nestor J.