Reversible interconversion of two forms of a valyl-tRNA synthetase-containing protein complex.
Reversible interconversion of two forms of a valyl-tRNA synthetase-containing protein complex.
复制标题
两种形式的含缬氨酰-tRNA 合成酶的蛋白质复合物的可逆相互转化。
作者：
S. Black
当酵母酶复合体在室温的缓冲溶液中孵育时，Valyl-Transfer RNA合成酶活性和总蛋白在两种物理状态之间同步振荡。这一观察表明，一种调控过程将一些酶作为一个群体进行控制，这是一种迄今为止尚未认识到的综合功能。两种形式的络合物在每30秒从孵化溶液中提取的样品中通过其中一种形式的硫酸铵沉淀来分离。高浓度(50 MM)的谷胱甘肽和二硫苏糖醇可促进50%饱和硫酸铵可溶形态的形成。氧化的谷胱甘肽、二磷吡啶核苷酸、三磷吡啶核苷酸和中等浓度(0.1-1.0 mM)的汞硫醇结合剂使分布向可沉淀形式转变。这两种形式代表了功能和非功能的复杂结合酶，它们在氧化还原信号的响应下相互转换。
When an enzyme-containing complex from yeast was incubated in a buffered solution at room temperature, the valyl-transfer RNA synthetase activity and total protein oscillated synchronously between two physical states. This observation suggests a regulatory process that controls a number of enzymes as a group, an integrated function of a kind not heretofore recognized. The two forms of the complex were separated by ammonium sulfate precipitation of one of them in samples withdrawn from the incubated solution every 30 seconds. Glutathione and dithiothreitol in high concentrations (50 mM) enhance formation of the 50% saturated ammonium sulfate-soluble form. Oxidized glutathione, diphosphopyridine nucleotide, triphosphopyridine nucleotide, and a mercurial thiol binding agent in moderate concentrations (0.1 to 1.0 mM) shift the distribution toward the precipitable form. It is suggested that the two forms represent functional and nonfunctional complex-bound enzymes which are interconverted in response to oxidoreductive signals.