INOSITOL 1,4,5-TRISPHOSPHATE RECEPTORS - DISTINCT NEURONAL AND NONNEURONAL FORMS DERIVED BY ALTERNATIVE SPLICING DIFFER IN PHOSPHORYLATION
INOSITOL 1,4,5-TRISPHOSPHATE RECEPTORS - DISTINCT NEURONAL AND NONNEURONAL FORMS DERIVED BY ALTERNATIVE SPLICING DIFFER IN PHOSPHORYLATION
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DOI：
10.1073/pnas.88.7.2951
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发表时间：
1991-04-01
影响因子：
11.1
通讯作者：
ROSS, CA
中科院分区：
文献类型：
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作者：
DANOFF, SK;FERRIS, CD;ROSS, CA
我们用PCR方法从大鼠不同组织中鉴定出两种不同的1，4，5-三磷酸肌醇受体转录本。 较长的形式，对应于先前克隆的成年大鼠脑肌醇1，4，5-三磷酸受体，包含两个cAMP依赖性蛋白激酶磷酸化共有序列之间的120个核苷酸插入。 较短的形式（缺乏插入）在胎儿脑和外周组织中占主导地位，似乎代表一个非神经元受体，而较长的形式在成人脑中发现，似乎是专门的神经元。 cAMP依赖性蛋白激酶的磷酸化动力学和磷酸肽图谱对于从主要表达不同形式的转录物的组织中纯化的肌醇1，4，5-三磷酸受体不同。
We have identified two distinct transcripts of inositol 1,4,5-trisphosphate receptor by using the PCR on first-strand cDNAs from various rat tissues. The longer form, corresponding to the previously cloned adult rat brain inositol 1,4,5-trisphosphate receptor, contains a 120-nucleotide insert between the two cAMP-dependent protein kinase phosphorylation consensus sequences. The shorter form (lacking the insert) predominates in fetal brain and peripheral tissues and appears to represent a nonneuronal receptor, whereas the longer form is found in adult brain and appears to be exclusively neuronal. The phosphorylation kinetics by cAMP-dependent protein kinase and the phosphopeptide maps differ for inositol 1,4,5-trisphosphate receptors purified from tissues predominantly expressing different forms of the transcript.