Diversity and abundance of ammonia-oxidizing bacteria in eutrophic and oligotrophic basins of a shallow Chinese lake (Lake Donghu)
Diversity and abundance of ammonia-oxidizing bacteria in eutrophic and oligotrophic basins of a shallow Chinese lake (Lake Donghu)
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DOI：
10.1016/j.resmic.2009.01.003
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发表时间：
2009-04-01
影响因子：
2.6
通讯作者：
Zhou, Yi-Yong
中科院分区：
文献类型：
--
作者：
Chen, Guo-Yuan;Qiu, Shan-Lian;Zhou, Yi-Yong
采用传统培养方法和基于氨单加氧酶基因（amoA）的分子方法，研究了湖泊沉积物中β -变形菌门氨氧化细菌（AOB）的丰度和多样性。对中国一个浅水湖泊（东湖）按铵（NH4 +）浓度划分的富营养和贫营养湖区进行了采样。贫营养湖区的AOB数量显著较低，而富营养湖区则显著较高。此外，利用针对amoA的限制性片段长度多态性分析，在富营养湖区发现了10种限制性图谱，其中包括6种独特图谱，而在贫营养湖区观察到5种图谱，且只有1种独特的限制群。对AOB的系统发育分析表明，与亚硝化单胞菌属寡营养型（Nitrosomonas oligotropha）和尿素亚硝化单胞菌（Nitrosomonas ureae）相关的AOB以及亚硝化螺菌属（Nitrosospira）相关的AOB普遍存在；前者在富营养湖区占主导地位（87.2%），而后者在贫营养湖区占主导地位（65.5%）。此外，与欧洲亚硝化单胞菌（Nitrosomonas communis）相关的AOB仅在富营养湖区被检测到，且比例较小（3.2%）。这些结果表明，在不同营养状态的湖泊中，沉积物中的AOB存在显著的选择和适应性。（C）2009爱思唯尔马松公司。版权所有。
Classical cultivation and molecular methods based on the ammonia monooxygenase gene (amoA) were used to study the abundance and diversity of beta-proteobacterial ammonia-oxidizing bacteria (AOB) in lake sediments. The eutrophic and oligotrophic basins of a Chinese shallow lake (Lake Donghu), in terms of ammonium (NH4+) concentrations, were sampled. The AOB number was significantly lower in the oligotrophic basin, but significantly higher in the eutrophic basin. In addition, using restriction fragment length polymorphism targeting the amoA, ten restriction patterns including six unique ones were found in the eutrophic basin, while five patterns were observed in the oligotrophic basin with only one unique restriction group. Phylogenetic analysis for AOB revealed that Nitrosomonas oligotropha- and Nitrosomonas ureae-related AOB and Nitrosospira-affiliated AOB were ubiquitous; the former dominated in the eutrophic basin (87.2%), while the latter dominated in the oligotrophic basin (65.5%). Furthermore, Nitrosomonas communis-related AOB was only detected in the eutrophic basin, at a small proportion (3.2%). These results indicate significant selection and adaptation of sediment AOB in lakes with differing trophic status. (C) 2009 Elsevier Masson SAS. All rights reserved.