Hydroxyl radicals generated in the rat spinal cord at the level produced by impact injury induce cell death by necrosis and apoptosis: Protection by a metalloporphyrin
Hydroxyl radicals generated in the rat spinal cord at the level produced by impact injury induce cell death by necrosis and apoptosis: Protection by a metalloporphyrin
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DOI：
10.1016/j.neuroscience.2004.03.054
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发表时间：
2004-01-01
期刊：
影响因子：
3.3
通讯作者：
Liu, D
中科院分区：
文献类型：
--
作者：
Bao, F;Liu, D
我们先前测量了过氧化氢（H2 O2），羟基自由基（（OH）-O-），和催化铁在创伤性脊髓损伤（SCI）后增加。本研究旨在确定SCI是否升高了（OH）-O-水平。导致细胞死亡。通过在SCI后达到的浓度下施用H2 O2和Fe 2+来产生OH，每个通过横向插入脊髓灰质的单独的微透析纤维。（OH）-O-的持续时间。这代人模仿了SCI后其升高的持续时间。（OH）-O-后24 h，神经元和星形胶质细胞死亡。暴露，并通过对用甲酚紫染色或用抗神经元特异性烯醇化酶（抗NSE）和抗神经胶质细胞酸性蛋白（抗GFAP）化学染色的切片中沿纤维轨迹的存活细胞沿着计数来定量。用末端脱氧核苷酸转移酶介导的脱氧尿苷三磷酸-生物素缺口末端标记法（JUNEL）和抗NSE双染法检测神经元DNA片段化。使用单因素方差分析，然后进行Tukey检验，我们证明了（OH）-O-。在脊髓中产生的Cresyl Violet引起神经元的显著损失（P
We previously measured the time courses of hydrogen peroxide (H2O2), hydroxyl radical ((OH)-O-.), and catalytic iron increases following traumatic spinal cord injury (SCI). This study determines whether the SCI-elevated level of (OH)-O-. causes cell death. OH was generated by administering H2O2 and Fe2+ at the concentrations attained following SCI, each through a separate microdialysis fiber inserted laterally into the gray matter of the cord. The duration of (OH)-O-. generation mimics the duration of its elevation after SCI. The death of neurons and astrocytes was characterized at 24 h post-(OH)-O-. exposure and quantitated by counting surviving cells along the fiber track in sections stained with Cresyl Violet, or immunohistochemically stained with anti-neuron-specific enolase (anti-NSE) and anti-glial fibrillary acidic protein (antiGFAP). DNA fragmentation in neurons was characterized by double staining with terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate-biotin nick end labeling JUNEL) and anti-NSE. Using a one way ANOVA followed by the Tukey test, we demonstrated that (OH)-O-. generated in the cord induced significant losses of neurons in both Cresyl Violet (P