Effect of gga-miR-155 on cell proliferation, apoptosis and invasion of Marek's disease virus (MDV) transformed cell line MSB1 by targeting RORA
Effect of gga-miR-155 on cell proliferation, apoptosis and invasion of Marek's disease virus (MDV) transformed cell line MSB1 by targeting RORA
复制标题
gga-miR-155靶向RORA对马立克氏病病毒（MDV）转化细胞系MSB1细胞增殖、凋亡和侵袭的影响
DOI：
10.1186/s12917-020-2239-4
复制
发表时间：
2020-01-28
影响因子：
2.6
通讯作者：
Yu, Zu-Ling
中科院分区：
文献类型：
--
作者：
Ding, Ke;Yu, Zu-Hua;Yu, Zu-Ling
背景马立克氏病(MD)是由致癌的马立克氏病病毒(MDV)引起的一种高度传染性的禽类传染病，具有复杂的基础病理，涉及淋巴增生性肿瘤的形成。在大多数癌症中，microRNAs(MiRNAs)起癌基因或肿瘤抑制基因的作用。在感染MDV的鸡组织或淋巴细胞系中，gga-miR-155表达下调，尽管它在肿瘤发生中的确切作用尚不清楚。本研究的目的是分析gga-miR-155对MDV转化的淋巴细胞系MSB1的增殖、凋亡和侵袭力的影响，并阐明其作用机制。结果在MSB1细胞中，gga-miR-155的表达水平可通过特异性模拟物和抑制剂进行调控。而gga-miR-155过表达则促进细胞增殖，降低G1期细胞比例，减少细胞凋亡率，增加侵袭力。然而，其下调监管产生了相反的效果。此外，gga-miR-155直接靶向RORA基因并下调其在MSB1细胞中的表达。结论gga-miR-155通过靶向RORA基因，促进MDV转化的淋巴细胞系MSB1的增殖和侵袭，抑制细胞凋亡。
Background Marek's disease (MD) is caused by the oncogenic Marek's disease virus (MDV), and is a highly contagious avian infection with a complex underlying pathology that involves lymphoproliferative neoplasm formation. MicroRNAs (miRNAs) act as oncogenes or tumor suppressors in most cancers. The gga-miR-155 is downregulated in the MDV-infected chicken tissues or lymphocyte lines, although its exact role in tumorigenesis remains unclear. The aim of this study was to analyze the effects of gga-miR-155 on the proliferation, apoptosis and invasiveness of an MDV-transformed lymphocyte line MSB1 and elucidate the underlying mechanisms. Results The expression level of gga-miR-155 was manipulated in MSB1 cells using specific mimics and inhibitors. While overexpression of gga-miR-155 increased proliferation, decreased the proportion of G1 phase cells relative to that in S and G2 phases, reduced apoptosis rates and increased invasiveness. However, its downregulation had the opposite effects. Furthermore, gga-miR-155 directly targeted the RORA gene and downregulated its expression in the MSB1 cells. Conclusion The gga-miR-155 promotes the proliferation and invasiveness of the MDV-transformed lymphocyte line MSB1 and inhibits apoptosis by targeting the RORA gene.