Serotonin synchronises convergent extension of ectoderm with morphogenetic gastrulation movements in Drosophila
Serotonin synchronises convergent extension of ectoderm with morphogenetic gastrulation movements in Drosophila
复制标题
DOI：
10.1016/s0925-4773(99)00141-0
复制
发表时间：
1999-09-01
影响因子：
2.6
通讯作者：
Maroteaux, L
中科院分区：
文献类型：
--
作者：
Colas, JF;Launay, JM;Maroteaux, L
在果蝇原肠发育过程中，外胚层的收敛延伸是生殖带延伸所必需的。外胚层细胞内的粘附性异质性被认为触发了负责这种运动的细胞的嵌入。分割基因将通过建立细胞黏附特性的配对规则模式来施加这种异质性。我们先前报道，5-羟色胺受体(5-HT(2Dro))在推测的外胚层中以成对规则的模式表达。在这里，我们表明，5-羟色胺(2Dro)、表达和5-羟色胺合成的峰值恰好与外胚层会聚延伸的开始相吻合。缺乏5-羟色胺或5-羟色胺(2-Dro)受体的胃壁不能正常延伸胚带，外胚层的运动与内胚层和中胚层的形态发生运动不同步。与这种去同步化的开始相关的是外胚层内贴壁连接的亚细胞定位的改变。综上所述，这些数据强调了外胚层在果蝇原肠发育中的作用，并支持了5-羟色胺通过5-HT2Dro受体发出的信号会触发细胞粘附性变化的观点，而细胞粘附性是细胞嵌入所必需的。(C)1999 EIsevier Science爱尔兰有限公司。保留所有权利。
During Drosophila gastrulation, convergent extension of the ectoderm is required for germband extension. Adhesive heterogeneity within ectodermal cells has been proposed to trigger the intercalation of cells responsible for this movement. Segmentation genes would impose this heterogeneity by establishing a pair-rule pattern of cell adhesion properties. We previously reported that the serotonin receptor (5-ht(2Dro)) is expressed in the presumptive ectoderm with a pair-rule pattern. Here, we show that the peaks of 5-ht(2Dro), expression and serotonin synthesis coincide precisely with the onset of convergent extension of the ectoderm. Gastrulae genetically depleted of serotonin or the 5-ht(2Dro) receptor do not extend their germband properly, and the ectodermal movements becomes asynchronous with the morphogenetic movements in the endoderm and mesoderm. Associated with the beginning of this desynchronisation, is an altered subcellular localisation of adherens junctions within the ectoderm. Combined, these data highlight the role of the ectoderm in Drosophila gastrulation and support the notion that serotonin signalling through the 5-HT2Dro receptor triggers changes in cell adhesiveness that are necessary for cell intercalation. (C) 1999 EIsevier Science Ireland Ltd. All rights reserved.