Abundant expression of EBER1 small nuclear RNA in nasopharyngeal carcinoma. A morphologically distinctive target for detection of Epstein-Barr virus in formalin-fixed paraffin-embedded carcinoma specimens.
Abundant expression of EBER1 small nuclear RNA in nasopharyngeal carcinoma. A morphologically distinctive target for detection of Epstein-Barr virus in formalin-fixed paraffin-embedded carcinoma specimens.
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发表时间：
1991-06
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通讯作者：
Tzyy Choou Wu;Risa B. Mann;Jonathan I. Epstein;E. Macmahon;W. Lee;Patricia Charache;S. Hayward;Robert J. Kurman;Gary S. Hayward;R. Ambinder
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作者：
Tzyy Choou Wu;Risa B. Mann;Jonathan I. Epstein;E. Macmahon;W. Lee;Patricia Charache;S. Hayward;Robert J. Kurman;Gary S. Hayward;R. Ambinder
eb病毒（EBV）基因组在恶性肿瘤中的高度限制性表达，限制了适用于福尔马林固定石蜡包埋癌标本的EBV检测方法的使用。在EBV转化的淋巴细胞中，非常短的非蛋白质编码EBV转录本（EBERs）的表达量比其他EBV潜伏期转录本高得多（每个细胞10(7)个拷贝）。利用3H核糖探针，作者证实了EBER1在鼻咽癌（npc）和腮腺唾液腺中的表达。对EBER1表达的识别得益于杂交强度及其特征形态（核仁保留）。EBER1在粘膜表面（口咽、子宫颈）产生的其他上皮恶性肿瘤中未被证实表达，这些肿瘤已检测到EBV的脱落。在没有背景的情况下，用地高辛标记的探针重复研究鼻鼻癌标本，在12小时的过程中产生具有亚细胞形态细节的杂交信号。因此，EBER1转录物是福尔马林固定石蜡包埋癌标本中EBV原位杂交检测的合适靶点。
The highly restricted expression of the Epstein-Barr virus (EBV) genome in malignancy has limited the use of EBV detection methods applicable to formalin-fixed paraffin-embedded carcinoma specimens. In EBV-transformed lymphocytes very short nonprotein coding EBV transcripts (EBERs) are expressed in much higher abundance (10(7) copies per cell) than other EBV latency transcripts. Using a 3H riboprobe, the authors demonstrated EBER1 expression in NASOPHARYNGEAL CARCINOMAS (NPCs) as well as in parotid salivary gland. Recognition of EBER1 expression was facilitated by the intensity of hybridization and its characteristic morphology (nuclear with nucleolar sparing). EBER1 expression was not demonstrated in other epithelial malignancies arising from mucosal surfaces (oropharynx, uterine cervix) from which EBV shedding has been detected. Repeat study of the NPC specimens with digoxigenin-labeled probe yielded hybridization signal with subcellular morphologic detail and without background in a 12-hour procedure. Thus the EBER1 transcript is an appropriate target for in situ hybridization detection of EBV in formalin-fixed paraffin-embedded carcinoma specimens.