Super-kdrmutationM918Land multiple cytochrome P450s associated with the resistance ofRhopalosiphum padito pyrethroid
Super-kdrmutationM918Land multiple cytochrome P450s associated with the resistance ofRhopalosiphum padito pyrethroid
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超k突变M918和多个细胞色素P450与拟除虫菊酯抗性相关
DOI：
10.1002/ps.5829
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发表时间：
2020
影响因子：
4.1
通讯作者：
Chen Maohua
中科院分区：
文献类型：
--
作者：
Wang Kang;Bai Jiaoyang;Zhao Junning;Su Sha;Liu Lang;Han Zhaojun;Chen Maohua
背景：稻谷蚜是世界范围内危害谷类作物的重要害虫。拟除虫菊酯，包括氯氟氰菊酯，已被广泛用于控制疟疾。在田野里。本文研究了r的抗性水平。稻田蚜虫对氯氟氰菊酯的抗性，并分析了蚜虫对拟除虫菊酯类杀虫剂抗性的生化和分子机制。结果采集了抗氯氟氰菊酯（lambda - cyhalthryn）田间种群（JY），在种群中鉴定出电压门控钠通道（VGSC）的一个超级突变M918L。随后，通过选择使用氯氟氰菊酯的田间种群，建立了抗氯氟氰菊酯品系（LC - R）。所有的人。padiLC‐R菌株在VGSC IIS4-IIS6区出现M918L杂合突变。检测了LC - R菌株对9种杀虫剂的交叉抗性谱。协同和酶活性研究表明，细胞色素P450单加氧酶在这种抗性中起重要作用。进一步的基因表达分析表明，LC‐R菌株与敏感菌株相比，7个P450基因显著上调。结论新疆地区已发现拟除虫菊酯类杀虫剂的田间进化抗性。padi。在一个重要的小麦种植区获得的田间样品中首次记录了VGSC中的M918L（超级kdr）突变。超脱氢突变以及由P450基因介导的代谢抗性被确定为对r产生抗性的原因。padi。©2020化学工业协会
BACKGROUNDRhopalosiphum padiis an important pest affecting cereal crops worldwide. Pyrethroid, including lambda‐cyhalothrin, has been widely used to controlR. padiin the field. This work investigated the resistance levels ofR. padifield populations to lambda‐cyhalothrin, and analysed biochemical and molecular mechanisms of aphid resistance to the insecticide pyrethroid.RESULTSA lambda‐cyhalothrin‐resistant field population (JY) was sampled, and a super‐kdrmutation, M918L, in the voltage‐gated sodium channel (VGSC) was identified in the population. The lambda‐cyhalothrin‐resistant strain (LC‐R) was subsequently established by selecting the field population with lambda‐cyhalothrin. All individuals of theR. padiLC‐R strain showed the M918L heterozygous mutation in the VGSC IIS4–IIS6 region. Cross‐resistance profiles of the LC‐R strain to nine insecticides were detected. Both synergistic and enzyme activity studies indicated that cytochrome P450 monooxygenase played an important role in this resistance. Further gene expression analysis showed that seven P450 genes were significantly upregulated in the LC‐R strain compared with the susceptible strain.CONCLUSIONField‐evolved resistance to pyrethroid insecticides has been found inR. padi. The M918L (super‐kdr) mutation in the VGSC was documented for the first time in field samples obtained from an important wheat‐growing area. The super‐kdrmutation, as well as metabolic resistance mediated by P450 genes, was determined to contribute to the lambda‐cyhalothrin resistance inR. padi. © 2020 Society of Chemical Industry