Sodium dodecyl sulfate decorated Legionella pneumophila for enhanced detection with a GaAs/AlGaAs nanoheterostructure biosensor
Sodium dodecyl sulfate decorated Legionella pneumophila for enhanced detection with a GaAs/AlGaAs nanoheterostructure biosensor
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DOI：
10.1016/j.snb.2019.127007
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发表时间：
2020-02-01
影响因子：
8.4
通讯作者：
Dubowski, J. J.
中科院分区：
文献类型：
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作者：
Aziziyan, M. R.;Hassen, W. M.;Dubowski, J. J.
与细菌菌株相关的净电荷主要由其表面可用官能团的数量定义，我们观察到它可以确定电荷传感生物传感器的检测限。我们通过结合带负电荷的十二烷基硫酸钠 (SDS) 分子研究了细菌电荷变化的动态范围，目的是提高专为检测嗜肺军团菌而设计的电荷感应 GaAs/AlGaAs 纳米异质结构生物传感器的检测限。将这些细菌暴露于 0.02 mg/mL 的 SDS 溶液后，在 pH 7.4 下测量到嗜肺军团菌的 zeta 电位增加两倍。随后，可以检测到 10(3) CFU/mL 的 SDS 修饰和热灭活的嗜肺军团菌。这说明了细菌电荷在基于光腐蚀的 III-V 族半导体生物芯片运行中的基本作用。我们讨论了细菌与 SDS 相互作用的机制、用这种阴离子表面活性剂装饰细菌的关键方面以及负责电荷转移的通道。
The net electric charge associated with a bacterial strain is primarily defined by the number of available functional groups at its surface and we observed that it can determine the limit of detection of a charge-sensing biosensor. We have investigated the dynamic range of bacterial electric charge variations through binding negatively charged sodium dodecyl sulphate (SDS) molecules, with the objective of improving the detection limit of a charge-sensing GaAs/AlGaAs nanoheterostructure biosensor designed for detection of Legionella pneumophila. A two-fold increased zeta potential of L. pneumophila was measured at pH 7.4 following the exposure of these bacteria to an SDS solution at 0.02 mg/mL. Subsequently, it was possible to detect SDS decorated and heatinactivated L. pneumophila at 10(3) CFU/mL. This illustrates the fundamental role of the bacterial electric charge in the operation of photocorrosion-based III-V semiconductor biochips. We discuss the mechanisms of bacterial interaction with SDS, critical aspects of decorating bacteria with this anionic surfactant and the channels responsible for charge transfer.