A Novel cis Element Achieves the Same Solution as an Ancestral cis Element During Thiamine Starvation in Candida glabrata
A Novel cis Element Achieves the Same Solution as an Ancestral cis Element During Thiamine Starvation in Candida glabrata
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DOI：
10.1534/g3.119.400897
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发表时间：
2020-01-01
影响因子：
2.6
通讯作者：
Wykoff, Dennis D.
中科院分区：
文献类型：
--
作者：
Iosue, Christine L.;Gulotta, Anthony P.;Wykoff, Dennis D.
由于转录因子的限制，调控网络通常会聚集在非常相似的顺式序列上来驱动转录程序。为了确定顺式元件进化的约束损失的作用，我们研究了最近出现的硫胺素饥饿调节启动子在光滑念珠菌。该物种缺乏祖先的转录因子Thi 2，但仍然有转录因子Pdc 2，它调节硫胺素饥饿基因，使我们能够确定约束变化对新启动子的影响。我们在C中鉴定了两个不同的顺式元件。glabrata -一个存在于称为CgPMU 3的进化最近的基因中，另一个存在于其他硫胺素（THI）调节基因中。顺式元件的相互交换和S.将酿酒酵母转录因子结合位点插入到这些启动子中证明了这两个元件在功能上彼此不同。因此，这种对启动子功能的强加约束的丧失产生了新的顺式序列，表明反式约束的丧失可以产生具有相同输出的非收敛途径。
Regulatory networks often converge on very similar cis sequences to drive transcriptional programs due to constraints on what transcription factors are present. To determine the role of constraint loss on cis element evolution, we examined the recent appearance of a thiamine starvation regulated promoter in Candida glabrata. This species lacks the ancestral transcription factor Thi2, but still has the transcription factor Pdc2, which regulates thiamine starvation genes, allowing us to determine the effect of constraint change on a new promoter. We identified two different cis elements in C. glabrata - one present in the evolutionarily recent gene called CgPMU3, and the other element present in the other thiamine (THI) regulated genes. Reciprocal swaps of the cis elements and incorporation of the S. cerevisiae transcription factor-binding site into these promoters demonstrate that the two elements are functionally different from one another. Thus, this loss of an imposed constraint on promoter function has generated a novel cis sequence, suggesting that loss of trans constraints can generate a non-convergent pathway with the same output.