A novel microfluidic platform with stable concentration gradient for on chip cell culture and screening assays
A novel microfluidic platform with stable concentration gradient for on chip cell culture and screening assays
复制标题
一种具有稳定浓度梯度的新型微流体平台，用于芯片上细胞培养和筛选测定
DOI：
10.1039/c3lc50676b
复制
发表时间：
2013-01-01
期刊：
影响因子：
6.1
通讯作者：
Chen, Hong-Yuan
中科院分区：
文献类型：
--
作者：
Xu, Bi-Yi;Hu, Shan-Wen;Chen, Hong-Yuan
在这项工作中，一种新的微流控平台的细胞培养和分析的发展。在芯片上，静态细胞培养区域与动态流体营养供应结构相结合。细胞培养单元具有夹层结构，其中液体通道在顶部，细胞培养储器在中间，气体通道在底部。样品可以很容易地装载到容器中，并通过扩散与外部液体环境不断交换。由于流动方向垂直于储液器顶部的液体通道，因此储液器中的细胞被保护免受剪切力。通过将基本单元组装成阵列，可以产生稳定的浓度梯度。在芯片上建立了连续灌注和一次性灌注的细胞培养模型。在二维和三维培养模式下，贴壁细胞和悬浮细胞均在芯片上成功培养。培养后，回收捕获的细胞用于随后的测定。作为标准细胞培养和检测平台的竞争候选者，该芯片也适用于细胞毒性和细胞生长检测。
In this work a novel microfluidic platform for cell culture and assay is developed. On the chip a static cell culture region is coupled with dynamic fluidic nutrition supply structures. The cell culture unit has a sandwich structure with liquid channels on the top, the cell culture reservoir in the middle and gas channels on the bottom. Samples can be easily loaded into the reservoir and exchange constantly with the external liquid environment by diffusion. Since the flow direction is perpendicular to the liquid channel on the top of the reservoir, the cells in the reservoir are shielded from shear-force. By assembling the basic units into an array, a steady concentration gradient can be generated. Cell culture models both for continuous perfusion and one-off perfusion were established on the chip. Both adherent and suspended cells were successfully cultured on the chip in 2D and 3D culture modes. After culturing, the trapped cells were recovered for use in a later assay. As a competitive candidate for a standard cell culture and assay platform, this chip is also adaptable for cytotoxicity and cell growth assays.