Synthesis of short RNA fragments by the benzotriazolyl phosphotriester approach.
Synthesis of short RNA fragments by the benzotriazolyl phosphotriester approach.
复制标题
通过苯并三唑基磷酸三酯方法合成短 RNA 片段。
DOI：
10.1093/nar/11.23.8389
复制
发表时间：
1983
影响因子：
14.9
通讯作者：
J. H. Boom
中科院分区：
文献类型：
--
作者：
C. Wreesmann;A. Fidder;G. A. Marel;J. H. Boom
Analytical and experimental evidence will be presented to show that addition of a slight excess of 2-chlorophenyl-0,0-bis[1-benzotriazolyl]phosphate to a properly 2',5'-protected ribonucleoside gives an intermediate 3'-phosphotriester, which, after addition of a 2'-protected ribonucleoside, affords a dimer containing solely a 3'-5'-internucleotide linkage. The above phosphorylation procedure has been used in the synthesis of the pentamer UpApCpGpC.
