Primary culture of chicken hepatocytes in serum-free medium (pH 7.8) secreted albumin and transferrin for a long period in free gas exchange with the atmosphere
Primary culture of chicken hepatocytes in serum-free medium (pH 7.8) secreted albumin and transferrin for a long period in free gas exchange with the atmosphere
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DOI：
10.1016/s1357-2725(96)00076-3
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发表时间：
1996-12-01
影响因子：
4
通讯作者：
Sugano, T
中科院分区：
文献类型：
--
作者：
Fujii, M;Yoshino, I;Sugano, T
为了研究鸡的肝功能，我们对鸡肝细胞的原代培养进行了检查，发现了一种简单的自由空气交换长期培养方法。通过胶原酶灌注获得鸡肝细胞，并在无培养基的无血清L-15培养基（pH 7.8）中以单层形式在37℃下进行自由空气交换培养。通过ELISA测定培养基中白蛋白和转铁蛋白的量。将鸡肝细胞培养物在无血清L-15培养基(pH 7.8)中在37℃下自由空气交换维持20天。在培养早期，培养基中分泌的白蛋白量下降至较低水平；然而，随后从培养第9天到第17天显着增加。转铁蛋白的量在第 6 天之前保持恒定，然后随着进一步培养，转铁蛋白的量也显着增加。我们报道了一种简单的方法，用于在无血清 L-15 培养基（pH 7.8）中进行鸡肝细胞的简单单层培养，并在较长时间内进行自由空气交换。肝脏特异性功能的表达，即。培养1周后观察白蛋白和转铁蛋白的合成。版权所有 (C) 1996 爱思唯尔科学有限公司
To study the liver functions of chicken, we examined the primary culture of chicken hepatocytes, and found an easy method of long-term culture with free atmosphere exchange. Chicken hepatocytes were obtained by collagenase perfusion and cultured at 37 degrees C as a monolayer without substratum in serum-free L-15 medium (pH 7.8) with free atmosphere exchange. The amounts of albumin and transferrin in medium were assayed by ELISA. The culture of chicken hepatocytes was maintained in the serum-free L-15 medium (pH 7.8) at 37 degrees C with free atmosphere exchange for 20 days. The amount of albumin secreted in the medium decreased to low levels early in culture; however, this was followed by marked increase from day 9 to day 17 of culture. The amount of transferrin was constant until day 6, then it too increased considerably with further culture. We reported an easy method for the simple monolayer culture of chicken hepatocytes in serum-free L-15 medium (pH 7.8) with free atmosphere exchange over an extended period. Expression of liver-specific functions, viz. albumin and transferrin synthesis, was observed after 1 week of culture. Copyright (C) 1996 Elsevier Science Ltd