Analyzer-based imaging of spinal fusion in an animal model.
Analyzer-based imaging of spinal fusion in an animal model.
复制标题
动物模型中基于分析仪的脊柱融合成像。
DOI：
10.1088/0031-9155/53/10/011
复制
发表时间：
2008
影响因子：
3.5
通讯作者：
Chapman,LD
中科院分区：
文献类型：
--
作者：
Kelly,ME;Beavis,RC;Fiorella,David;Schultke,E;Allen,LA;Juurlink,BH;Zhong,Z;Chapman,LD
基于分析仪的成像（ABI）利用同步辐射源来产生准直的单色X射线。除了X射线吸收之外，该技术还使用折射和散射抑制来创建图像。ABI提供了比标准成像技术显著提高的对比度。将21只成年雄性Wistar大鼠分为4个实验组：（1）非损伤对照组，（2）单纯去皮质组，（3）去皮质加髂嵴植骨组和（4）去皮质加髂嵴植骨加棘突间钢丝组。在L5-6节段进行手术。分别于术后2、4、6周处死动物，切除脊柱肌肉块。通过（1）手动测试、（2）常规吸收X线摄影和（3）ABI评估样本是否存在融合。第6周时，ABI显示第1组和第2组中无骨融合证据，第3组和第4组受试者显示牢固或可能牢固融合。所有ABI图像中均不存在金属伪影。常规吸收X线片不能提供任何组中任何样本的移植物材料或融合块的诊断质量成像。基于同步加速器的ABI代表了一种新的成像技术，可用于评估小动物模型中的脊柱融合。与传统X线片相比，ABI可产生上级图像质量。
Analyzer-based imaging (ABI) utilizes synchrotron radiation sources to create collimated monochromatic x-rays. In addition to x-ray absorption, this technique uses refraction and scatter rejection to create images. ABI provides dramatically improved contrast over standard imaging techniques. Twenty-one adult male Wistar rats were divided into four experimental groups to undergo the following interventions:(1) non-injured control,(2) decortication alone,(3) decortication with iliac crest bone grafting and (4) decortication with iliac crest bone grafting and interspinous wiring. Surgical procedures were performed at the L5–6 level. Animals were killed at 2, 4 and 6 weeks after the intervention and the spine muscle blocks were excised. Specimens were assessed for the presence of fusion by (1) manual testing,(2) conventional absorption radiography and (3) ABI. ABI showed no evidence of bone fusion in groups 1 and 2 and showed solid or possibly solid fusion in subjects from groups 3 and 4 at 6 weeks. Metal artifacts were not present in any of the ABI images. Conventional absorption radiographs did not provide diagnostic quality imaging of either the graft material or fusion masses in any of the specimens in any of the groups. Synchrotron-based ABI represents a novel imaging technique which can be used to assess spinal fusion in a small animal model. ABI produces superior image quality when compared to conventional radiographs.