Rates and patterns of chromosomal evolution in Drosophila pseudoobscura and D. miranda
Rates and patterns of chromosomal evolution in Drosophila pseudoobscura and D. miranda
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DOI：
10.1534/genetics.105.054585
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发表时间：
2006-06-01
期刊：
影响因子：
3.3
通讯作者：
Charlesworth, Brian
中科院分区：
文献类型：
--
作者：
Bartolome, Carolina;Charlesworth, Brian
物种之间基因顺序的比较允许估计染色体进化的速度，因为他们从一个共同的祖先分歧。本文比较了拟暗果蝇（Drosophila pseudobscura）及其近缘种D.米兰达和远缘外群种D. melanogaster，利用D. Pseudobscura和D.与50个基因探针在黑腹果蝇中的原位杂交结果相似。米兰达我们没有发现D染色体间基因广泛转移的证据。米兰达D.米兰达和D. pseudobscura的比率远高于以前在果蝇中发现的比率，并接近线虫的比率，线虫是高等真核生物中比率最快的。此外，我们还发现D.具有最高水平的倒位多态性（Muller元件C）的pseudobscura染色体在染色体结构方面没有显示出异常快的进化速率，这表明这种经典的倒位多态性情况反映了选择而不是突变过程。在此基础上，我们提出了D. pseudobscuraC染色体，与目前文献报道的结果不同。我们还描述了一种新的方法，用于纠正重排，没有检测到一组有限的标记。
Comparisons of gene orders between species permit estimation of the rate of chromosomal evolution since their divergence from a common ancestor. We have compared gene orders on three chromosomes of Drosophila pseudoobscura with its close relative, D. miranda, and the distant outgroup species, D. melanogaster, by using the public genome sequences of D. pseudoobscura and D. melanogaster and similar to 50 in situ hybridizations of gene probes in D. miranda. We find no evidence for extensive transfer of genes among chromosomes in D. miranda. The rates of chromosomal rearrangements between D. miranda and D. pseudoobscura are far higher than those found before in Drosophila and approach those for nematodes, the fastest rates among higher eukaryotes. In addition, we find that the D. pseudoobscura chromosome with the highest level of inversion polymorphism (Muller's element C) does not show an unusually fast rate of evolution with respect to chromosome structure, suggesting that this classic case of inversion polymorphism reflects selection rather than mutational processes. On the basis of our results, we propose possible ancestral arrangements for the D. pseudoobscura C chromosome, which are different from those in the current literature. We also describe a new method for correcting for rearrangements that are not detected with a limited set of markers.