Transport and cleavage of bacterial pre-beta-lactamase by mammalian microsomes.
Transport and cleavage of bacterial pre-beta-lactamase by mammalian microsomes.
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DOI：
10.1083/jcb.96.4.1117
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发表时间：
1983-04
影响因子：
7.8
通讯作者：
Vasicek, T J
中科院分区：
文献类型：
--
作者：
Kronenberg, H M;Fennick, B J;Vasicek, T J
真核内质网和细菌内细胞膜使用惊人相似的机制将蛋白质从一个细胞区室运输到另一个细胞区室。使用哺乳动物连接的转录-翻译系统，我们在这里展示了犬胰腺微粒体准确地切割β-内酰胺酶（一种分泌的细菌蛋白）的信号序列。我们认为，细菌和真核细胞的蛋白质运输之间的明显差异可能只涉及一个深刻的保守机制的轻微调制。
Eucaryotic endoplasmic reticulum and bacterial inner cell membranes use strikingly similar mechanisms to transport proteins from one cellular compartment to another. Using a mammalian linked transcription-translation system, we show here that canine pancreatic microsomes accurately cleave off the signal sequence of β-lactamase, a secreted bacterial protein. We suggest that the apparent differences between bacterial and eukaryotic protein transport may involve only minor modulations of a profoundly conserved mechanism.