Receptors for the chemoattractants C5a and IL-8 are clustered on the surface of human neutrophils
Receptors for the chemoattractants C5a and IL-8 are clustered on the surface of human neutrophils
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DOI：
10.1177/002215549704501103
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发表时间：
1997-11-01
影响因子：
3.2
通讯作者：
Nelson, RD
中科院分区：
文献类型：
--
作者：
Gray, GD;Hasslen, SR;Nelson, RD
我们已经使用高分辨率场发射扫描电子显微镜与背散射电子成像检测免疫金标记的C5 a和白细胞介素-8（IL-8）受体的人血液中性粒细胞。受体用受体特异性抗体与缀合至免疫金的二抗组合标记。当中性粒细胞在“非活化”状态下分离时，这两种受体群体主要在细胞膜的非突出区域上的簇中表达。当这些细胞被C5 a和IL-8受体双重标记时，没有发现这些受体种类在一个共同簇中的混合。当中性粒细胞被分离为“激活"状态时，通过将血液与N-甲酰甲硫氨酰-亮氨酰-苯丙氨酸混合，可以看到细胞在其前极被拉长和褶皱，但C5 a受体没有分散或重新分布在肽激活的细胞表面上。由转染的小鼠L-细胞成纤维细胞表达的人C5 a受体的分布的分析显示C5 a受体是成簇的，但在细胞表面的非投射和投射域上表达。这些观察结果提供了新的信息的地形表达的白细胞受体参与指导细胞迁移。
We have used high-resolution field emission scanning electron microscopy with backscatter electron imaging to detect immunogold-labeled C5a and interleukin-8 (IL-8) receptors on human blood neutrophils. The receptors were labeled with receptor-specific antibodies in combination with secondary antibody conjugated to immunogold. When neutrophils were isolated in a ''nonactivated'' state, both of these receptor populations were expressed primarily in clusters on nonprojecting domains of the cell membrane. When these cells were double labeled for C5a and IL-8 receptors, intermixing of these receptor species in a common cluster was not found. When neutrophils were isolated in an ''activated'' state, by mixing the blood with N-formylmethionyl-leucyl-phenylalanine, the cells were seen to be elongated and ruffled at their anterior pole, but the C5a receptors did not disperse or redistribute on the surface of the peptide-activated cells. Analysis of the distribution of human C5a receptors expressed by transfected mouse L-cell fibroblasts showed the C5a receptors to be clustered, but expressed on nonprojecting and projecting domains of the cell surface. These observations provide new information on the topographical expression of leukocyte receptors involved in directing cell migration.